REGIV as a potential biomarker for peritoneal dissemination in gastric adenocarcinoma
REGIV as a potential biomarker for peritoneal dissemination in gastric adenocarcinoma
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DOI：
10.1002/jso.22021
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发表时间：
2012-02-01
影响因子：
2.5
通讯作者：
Doki, Yuichiro
中科院分区：
文献类型：
--
作者：
Moon, Jeong-Ho;Fujiwara, Yoshiyuki;Doki, Yuichiro
本研究旨在探讨再生胰岛衍生家族成员4（REGIV）在手术切除的胃肿瘤中的临床意义。还评估了REGIV作为胃癌生物标志物的潜力，包括其对预后和手术后复发的预测价值。方法：采用免疫组织化学方法评估手术切除标本中REGIV表达状态的临床意义。应用以REGIV mRNA为靶点的转录-逆转录协同反应（TRC）定量遗传学诊断方法预测胃癌腹膜复发。免疫组化结果显示，85例REGIV阳性（52.5%），与弥漫型组织病理学显著相关（P 0.001）、晚期T分期（P 0.022）和频繁腹膜复发（P = 0.009）。多因素分析表明，晚期T分期（P < 0.001）和REGIV表达（P = 0.034）是腹膜无复发生存的独立预后因素。REGIV蛋白在大多数腹膜肿瘤中过表达（93.8%）。结论：REGIV基因过表达在原发性胃肿瘤中普遍存在，可作为弥漫型组织病理学和腹膜播散的潜在指标。通过TRC方法评估，REGIV mRNA的过表达是根治性切除术后腹膜复发的潜在合适标志物。《肿瘤外科杂志》2012; 105：189-194. (C)2011 Wiley Periodicals，Inc.
Background This study examined the clinical significance of regenerating islet-derived family member 4 (REGIV) in surgically resected gastric tumors. The potential of REGIV as a biomarker in gastric cancer was also assessed including its predictive value for prognosis and recurrence after surgery.Methods: Immunohistochemistry was performed to assess the clinical significance of REGIV expression status in surgically resected specimens. The quantitative genetic diagnostic method, transcription-reverse transcription concerted reaction (TRC) that targeted REGIV mRNA was applied for prediction of peritoneal recurrence in gastric cancer.Results: Positive immunostaining for REGIV was observed in 85 cases (52.5%), and correlated significantly with diffuse type histopathology (P 0.001), advanced T stage (P 0.022), and frequent peritoneal recurrence (P = 0.009). Multivariate analysis identified advanced T stage (P < 0.001) and REGIV expression (P = 0.034) as independent prognostic factors for peritoneal recurrence-free survival. Overexpression of REGIV protein was evident in the majority of peritoneal tumors (93.8%). REGIV mRNA assessed by TRC could be a predictive marker for peritoneal recurrence after curative operation.Conclusions: REGIV overexpression is common in primary gastric tumors and a potentially suitable marker of diffuse type histopathology and peritoneal dissemination. Overexpression of REGIV mRNA, assessed by the TRC method, is a potentially suitable marker of peritoneal recurrence after curative resection. J. Surg. Oncol. 2012; 105: 189-194. (C) 2011 Wiley Periodicals, Inc.