XPB mediated retroviral cDNA degradation coincides with entry to the nucleus.
XPB mediated retroviral cDNA degradation coincides with entry to the nucleus.
复制标题
XPB 介导的逆转录病毒 cDNA 降解与进入细胞核同时发生。
DOI：
10.1016/j.virol.2010.11.016
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Fishel,Richard
中科院分区：
文献类型：
--
作者：
Yoder,KristineE;Roddick,William;Hoellerbauer,Pia;Fishel,Richard
逆转录病毒必须将它们的cDNA整合到宿主染色体上，但是逆转录病毒cDNA的显著部分在整合之前被降解。XPB和XPD是介导基础转录和DNA核苷酸切除修复的TFIIH复合物的一部分。当XPB或XPD发生突变时，逆转录病毒感染会增加。在这里，我们表明，抑制mRNA或蛋白质的合成不影响HIV cDNA的积累，这表明TFIIH转录活性是不需要降解。与cDNA稳定性有关的其他宿主因子不是XPB和XPD降解途径的组分。尽管XPB或XPD突变细胞中逆转录病毒cDNA的增加与整合前病毒的增加相关，但整合前复合物的整合效率不受影响。最后，HIV和MMLV cDNA降解似乎与核输入一致。这些结果表明，TFIIH介导的cDNA降解是针对逆转录病毒感染的核宿主防御。
Retroviruses must integrate their cDNA to a host chromosome, but a significant fraction of retroviral cDNA is degraded before integration. XPB and XPD are part of the TFIIH complex which mediates basal transcription and DNA nucleotide excision repair. Retroviral infection increases when XPB or XPD are mutant. Here we show that inhibition of mRNA or protein synthesis does not affect HIV cDNA accumulation suggesting that TFIIH transcription activity is not required for degradation. Other host factors implicated in the stability of cDNA are not components of the XPB and XPD degradation pathway. Although an increase of retroviral cDNA in XPB or XPD mutant cells correlates with an increase of integrated provirus, the integration efficiency of pre-integration complexes is unaffected. Finally, HIV and MMLV cDNA degradation appears to coincide with nuclear import. These results suggest that TFIIH mediated cDNA degradation is a nuclear host defense against retroviral infection.