Structural analysis of the 17q22-23 amplicon identifies several independent targets of amplification in breast cancer cell lines and tumors.
Structural analysis of the 17q22-23 amplicon identifies several independent targets of amplification in breast cancer cell lines and tumors.
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发表时间：
2001-07
期刊：
影响因子：
11.2
通讯作者：
Guojun Wu;Colleen S. Sinclair;Shannon R. Hinson;James N. Ingle;Patrick C. Roche;F. Couch
中科院分区：
文献类型：
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作者：
Guojun Wu;Colleen S. Sinclair;Shannon R. Hinson;James N. Ingle;Patrick C. Roche;F. Couch
最近在染色体 17q22-23 上发现了乳腺肿瘤中一个新的扩增区域。为了确定扩增过程所针对的区域中的癌基因，我们确定了乳腺癌细胞系和肿瘤中扩增子的结构。建立了大约 3.5 Mb 区域的物理和转录图谱，并用作通过 Southern 印迹和荧光原位杂交在该区域内进行拷贝数分析的基础。在乳腺癌细胞系和乳腺肿瘤中发现了七个特定且独立的扩增最大值。我们提出了 FLJ21316 和 Hs.6649 基因的相关扩增和过表达研究，表明这些候选基因作为扩增依赖性癌基因的作用。
A novel region of amplification in breast tumors has recently been identified on chromosome 17q22-23. In an effort to identify the oncogenes in the region that are targeted by the amplification process, we determined the structure of the amplicon in breast cancer cell lines and tumors. Physical and transcription maps of the approximately 3.5-Mb region were established and used as the basis for copy number analysis within the region by Southern blot and fluorescence in situ hybridization. Seven specific and independent amplification maxima were identified in breast cancer cell lines and breast tumors. We present correlative amplification and overexpression studies for the FLJ21316 and Hs.6649 genes suggesting a role for these candidates as amplification-dependent oncogenes.