AGE-DEPENDENCE OF MURINE SPERMATOGENESIS
AGE-DEPENDENCE OF MURINE SPERMATOGENESIS
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DOI：
10.1515/znc-1995-3-421
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发表时间：
1995-03-01
期刊：
影响因子：
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通讯作者：
HACKERKLOM, UB
中科院分区：
文献类型：
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作者：
HACKERKLOM, UB
研究小鼠精子发生功能与年龄的关系。因此，在DAPI染色DNA后，用流式细胞术分析每组0至26月龄至少10只NMRI小鼠的睾丸样本。这项研究的目的是能够解释年龄对老鼠的影响。在11周龄至16个月的小鼠中，睾丸重量和不同睾丸细胞类型的频率对精子发生没有影响。从16个月开始，精子发生功能有降低的趋势：圆形精子的频率减少。此外，随着年龄的增长，长形精子中染色质弥散度增加。因此，老年小鼠（bb ~ 16个月）不宜再用于辐射和药物效应等实验。异常二倍体精子的出现频率不随年龄增长而增加。
The dependence of spermatogenesis function on murine age shall be investigated. Thus, testicular samples of at least 10 NMRI mice per group aged 0 to 26 months are analysed by flow cytometry after staining the DNA with DAPI. The aim of this study is to be able to account for the influence of age on mice.There are no changes in spermatogenesis in mice aged 11 weeks up to 16 months with respect to testis weight and to the frequency of different testicular cell types. From 16 months onwards, there is a tendency to a reduced spermatogenesis function: The frequency of round spermatids is decreased. In addition, there is an increased chromatin dispersion in elongated spermatids with age. Thus, older mice (>16 months) should not be used for experiments e.g. on radiation and drug effects any more. The frequency of abnormal diploid spermatozoa does not increase with age.