Sponge paleogenomics reveals an ancient role for carbonic anhydrase in skeletogenesis
Sponge paleogenomics reveals an ancient role for carbonic anhydrase in skeletogenesis
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DOI：
10.1126/science.1141560
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发表时间：
2007-06-29
期刊：
影响因子：
56.9
通讯作者：
Woerheide, Gert
中科院分区：
文献类型：
--
作者：
Jackson, Daniel J.;Macis, Luciana;Woerheide, Gert
海绵（门Porifera）是古生代和中生代的多产造礁生物，类似于5.42至6500万年前。这些古老的动物从后生动物最后的共同祖先那里继承了第一个多细胞骨骼形成工具包的组成部分。利用古基因组学方法，包括基因和蛋白质表达技术以及系统发育重建，我们发现该工具包的一个分子成分是α -碳酸酐酶（α - cas）的前体，α -碳酸酐酶是现存动物在各种基本生理过程中使用的一个基因家族。我们使用了从中生代存活下来的珊瑚demosponge Astrosclera willeyana“活化石”，为生物钙化能力的进化提供了深入的见解，并表明α - ca家族从一个单一的祖先基因扩展到海绵和真生动物的几个独立的基因复制事件。
Sponges (phylum Porifera) were prolific reef-building organisms during the Paleozoic and Mesozoic similar to 542 to 65 million years ago. These ancient animals inherited components of the first multicellular skeletogenic toolkit from the last common ancestor of the Metazoa. Using a paleogenomics approach, including gene- and protein-expression techniques and phylogenetic reconstruction, we show that a molecular component of this toolkit was the precursor to the alpha-carbonic anhydrases (alpha-CAs), a gene family used by extant animals in a variety of fundamental physiological processes. We used the coralline demosponge Astrosclera willeyana, a "living fossil" that has survived from the Mesozoic, to provide insight into the evolution of the ability to biocalcify, and show that the alpha-CA family expanded from a single ancestral gene through several independent gene- duplication events in sponges and eumetazoans.