Relationship between lipid composition, frequency of ethanol-induced respiratory deficient mutants, and ethanol tolerance in Saccharomyces cerevisiae
Relationship between lipid composition, frequency of ethanol-induced respiratory deficient mutants, and ethanol tolerance in Saccharomyces cerevisiae
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DOI：
10.1046/j.1365-2672.1999.00793.x
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发表时间：
1999-06-01
影响因子：
4
通讯作者：
Arneborg, N
中科院分区：
文献类型：
--
作者：
Chi, Z;Arneborg, N
研究了两株具有不同乙醇耐受性的酿酒酵母（Saccharomycescerevisiae）中乙醇诱导的呼吸缺陷突变株的频率和脂质组成。乙醇耐受性更强的菌株比乙醇耐受性更弱的菌株表现出更低的乙醇诱导的呼吸缺陷突变体频率。此外，乙醇耐受性更强的菌株含有更高的麦角甾醇/磷脂比，更高比例的磷脂酰胆碱，更低比例的磷脂酰乙醇胺，更高的掺入总磷脂中的长链脂肪酸，和稍高比例的不饱和脂肪酸在总磷脂中比乙醇耐受性较低的菌株。这些结果表明，脂质组成，乙醇诱导的呼吸缺陷突变体的频率和酒精耐受性之间的明确关系。啤酒。这种关系的一种可能的解释进行了讨论。
The frequency of ethanol-induced respiratory deficient mutants and lipid composition in two Saccharomyces cerevisiae strains showing different degrees of ethanol tolerance were investigated. The more ethanol-tolerant strain exhibited a lower frequency of ethanol-induced respiratory deficient mutants than the less ethanol-tolerant strain. In addition, the more ethanol-tolerant strain contained a higher ergosterol/ phospholipid ratio, a higher proportion of phosphatidylcholine, a lower proportion of phosphatidylethanolamine, a higher incorporation of long-chain fatty acids in total phospholipids, and a slightly higher proportion of unsaturated fatty acids in total phospholipids than the less ethanol-tolerant strain. These results show a clear relationship between the lipid composition, the frequency of ethanol-induced respiratory deficient mutants, and the ethanol tolerance of S. cerevisiae. A possible explanation of this relationship is discussed.