The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants1[OPEN]
The Role of Plastidic Trigger Factor Serving Protein Biogenesis in Green Algae and Land Plants1[OPEN]
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塑料触发因子在绿藻和陆地植物中服务蛋白质生物发生的作用1[打开]
DOI：
10.1104/pp.18.01252
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发表时间：
2019
期刊：
影响因子：
7.4
通讯作者：
Willmund
中科院分区：
文献类型：
--
作者：
Gotsmann;Westrich;Trösch;Christmann;Zimmer;Mühlhaus;Sommer;Schroda;Keller;Möhlmann;Willmund
叶绿体中的生化过程几乎对所有生命形式都很重要。蛋白质稳态的严格调节和由进口和本地合成的亚基组成的蛋白质复合物的协调组装对于质体功能至关重要。蛋白质生物发生需要共翻译分子伴侣的作用。其中一种伴侣分子是触发因子（TF），已知它可以共翻译结合细菌中大多数新合成的蛋白质，从而帮助它们正确折叠和成熟。然而，人们对叶绿体中这些过程是如何调节的仍然知之甚少。我们在此报告绿藻（莱茵衣藻）和拟南芥（Arabidopsis thaliana）中叶绿体定位转录因子（TIG1）的功能研究。我们证明叶绿体 TIG1 进化为专门的伴侣。与其他在功能上可与原核对应物互换的质体伴侣不同，TIG1 无法补充大肠杆菌中广泛作用的直系同源物。虽然细菌和质体 TF 之间的一般伴侣特性（例如防止聚集或底物识别）似乎是保守的，但植物 TIG1 的不同之处在于仅与相对较小的翻译核糖体群体相关联。此外，质体 TIG1 水平的降低会导致蛋白质生物发生失调，但代价是翻译增加，从而破坏叶绿体能量家庭。这表明 TIG1 在叶绿体蛋白质生物发生中发挥核心作用。
Biochemical processes in chloroplasts are important for virtually all life forms. Tight regulation of protein homeostasis and the coordinated assembly of protein complexes, composed of both imported and locally synthesized subunits, are vital to plastid functionality. Protein biogenesis requires the action of cotranslationally acting molecular chaperones. One such chaperone is trigger factor (TF), which is known to cotranslationally bind most newly synthesized proteins in bacteria, thereby assisting their correct folding and maturation. However, how these processes are regulated in chloroplasts remains poorly understood. We report here functional investigation of chloroplast-localized TF (TIG1) in the green alga (Chlamydomonas reinhardtii) and the vascular land plant Arabidopsis (Arabidopsis thaliana). We show that chloroplastic TIG1 evolved as a specialized chaperone. Unlike other plastidic chaperones that are functionally interchangeable with their prokaryotic counterpart, TIG1 was not able to complement the broadly acting ortholog inEscherichia coli.Whereas general chaperone properties such as the prevention of aggregates or substrate recognition seems to be conserved between bacterial and plastidic TFs, plant TIG1s differed by associating with only a relatively small population of translating ribosomes. Furthermore, a reduction of plastidic TIG1 levels leads to deregulated protein biogenesis at the expense of increased translation, thereby disrupting the chloroplast energy household. This suggests a central role of TIG1 in protein biogenesis in the chloroplast.
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影响因子：
6
作者：
Hillaire MLB;Lawrence P;Lagrange B
通讯作者：
Lagrange B
影响因子：
64.5
作者：
Teter, SA;Houry, WA;Hartl, FU
通讯作者：
Hartl, FU
来自绿藻莱茵衣藻的 Rubisco 的第一个晶体结构*
影响因子：
4.8
作者：
T. C. Taylor;A. Backlund;Karin Bjorhall;R. J. Spreitzer;I. Andersson
通讯作者：
I. Andersson
影响因子：
5.1
作者：
Rütgers M;Muranaka LS;Mühlhaus T;Sommer F;Thoms S;Schurig J;Willmund F;Schulz-Raffelt M;Schroda M
通讯作者：
Schroda M
影响因子：
8
作者：
Huang, GC;Li, ZY;Fischer, G
通讯作者：
Fischer, G