Glucose-responsive gel from phenylborate polymer and poly(vinyl alcohol): Prompt response at physiological pH through the interaction of borate with amine group in the gel
Glucose-responsive gel from phenylborate polymer and poly(vinyl alcohol): Prompt response at physiological pH through the interaction of borate with amine group in the gel
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DOI：
10.1023/a:1012033718302
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发表时间：
1997-03-01
影响因子：
3.7
通讯作者：
Sakurai, Y
中科院分区：
文献类型：
--
作者：
Hisamitsu, I;Kataoka, K;Sakurai, Y
目的。基于苯基硼酸基聚合物与聚乙烯醇之间的络合作用，设计葡萄糖响应性凝胶。具体地说，通过苯基硼酸酯与氨基的相互作用，在生理pH下获得了高的葡萄糖敏感性。合成了间丙烯酰胺基苯基硼酸、N，N-二甲氨基丙基丙烯酰胺(DMAPAA)和N，N-二甲基丙烯酰胺三元共聚物。在三元共聚物中引入了DMAPAA，以稳定生理pH下的苯基硼酸酯-多元醇络合物。通过粘度和紫外光谱的测定，评价了氨基对络合物稳定性的影响。络合速度与三元共聚物中苯基硼酸酯和DMAPAA的含量有很好的相关性。随着络合速率的增加，UV差示光谱测量表明，由于与DMAPAA的相互作用，苯基硼酸在三元共聚物中的电离作用得到了促进。此外，随着葡萄糖浓度的变化，络合速率发生了灵敏的变化，这与不含DMAPAA的聚合物对葡萄糖的低敏感性形成了鲜明的对比。在苯基硼酸酯聚合物中引入氨基可以有效地提高其络合能力和在生理pH下的葡萄糖响应性。这些结果表明，这种复合凝胶系统在设计自我调节的胰岛素释放装置方面是可行的。
Purpose. To design glucose-responsive gels based on the complexation between polymers having phenylboronic acid groups and poly (vinyl alcohol). Specifically, high-glucose sensitivity at physiological pH was achieved through the interaction of phenylborate with amino groups.Method. Terpolymers of m-acrylamidophenylboronic acid, N,N-dimethylaminopropylacrylamide (DMAPAA), and N,N-dimethylacrylamide were prepared. DMAPAA was introduced in the terpolymer to stabilize phenylborate-polyol complex at physiological pH. The effect of amino groups on complex stabilization was estimated from viscosity as well as UV spectrum measurements.Results. A good correlation was observed between complexation rate and fraction of phenylborate as well as DMAPAA in the terpolymers. In line with this increased complexation rate, UV difference spectra measurement revealed that ionization of phenylboronic acid was facilitated in the terpolymer due to the interaction with DMAPAA. Further, sensitive change in the complexation rate was demonstrated with a variation in glucose concentration, which is in sharp contrast with the poor glucose-sensitivity of the polymer without DMAPAA.Conclusions. The introduction of an amino group into phenylborate polymers was quite effective for increasing the complexation ability and the glucose responsivity at physiological pH. These results suggest the feasibility of this complex-gel system in designing a self-regulated insulin-releasing device.