Inhibition of Adenovirus Transformation in Vitro by AAV-1 1
Inhibition of Adenovirus Transformation in Vitro by AAV-1 1
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DOI：
10.3181/00379727-140-36397
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发表时间：
1972-05
影响因子：
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通讯作者：
B. Casto;C. Goodheart
中科院分区：
文献类型：
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作者：
B. Casto;C. Goodheart
为了获得关于AAV抑制腺病毒转化的信息，用恒定量的猿猴腺病毒SA 7或SV 11，或用人腺病毒12型和不同的AAV-1多样性同时接种仓鼠胚胎细胞培养物。当加入9至10个空斑抑制单位（PIU）/细胞的AAV-1时，SV 11或Ad 12对转化细胞灶的诱导被抑制超过80%。将转化细胞灶减少50%所需的AAV-1的量范围为SV 11和Ad 12的1.5至2.5 PIU/细胞至SA 7的5至7 PIU/细胞。剂量-反应数据的分析表明，1单位的AAV-1/细胞足以抑制Ad 12和SV 11的转化。SA 7转化对抑制的相对抗性似乎最好解释为需要多于1个抑制单位/细胞的AAV-1。
Summary To obtain information on inhibition of adenovirus transformation by AAV, cultures of hamster embryo cells were inoculated simultaneously with constant amounts of simian adenoviruses SA7 or SV11, or with human adenovirus type 12 and with varying multiplicities of AAV-1. Induction of transformed cell foci by SV11 or Ad12 was inhibited by more than 80% when 9 to 10 plaque inhibiting units (PIU)/cell of AAV-1 were added. The amount of AAV-1 necessary to reduce transformed cell foci by 50% ranged from 1.5 to 2.5 PIU/cell for SV11 and Ad12 to 5 to 7 PIU/cell for SA7. Analysis of the dose-response data indicates that 1 unit of AAV-1/cell is sufficient to inhibit transformation by Ad 12 and SV11. The relative resistance of SA7 transformation to inhibition seems best explained as a requirement for more than 1 inhibiting unit/cell of AAV-1.