Apocynum venetum Leaf Attenuates Myocardial Ischemia/Reperfusion Injury by Inhibiting Oxidative Stress
Apocynum venetum Leaf Attenuates Myocardial Ischemia/Reperfusion Injury by Inhibiting Oxidative Stress
复制标题
罗布麻叶提取物通过抑制氧化应激减轻心肌缺血/再灌注损伤
DOI：
10.1142/s0192415x15500056
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发表时间：
2015-01-01
影响因子：
5.7
通讯作者：
Li, Rong
中科院分区：
文献类型：
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作者：
Wang, Wenqing;Liang, Xiangyan;Li, Rong
据报道，中国草药罗布麻具有神经保护作用。然而，是否罗布麻叶提取物（AVLE）保护缺血心肌仍然是难以捉摸的。本研究旨在观察AVLE预处理对心肌缺血/再灌注损伤的影响，并探讨其可能的机制。将大鼠用AVLE（500 mg/kg/d，o.g.）或蒸馏水，每天一次，持续一周。随后，所有动物均进行30 min心肌缺血，随后再灌注4 h。AVLE预处理1周可明显改善MI/R后心功能。AVLE可减少再灌注后心肌梗死面积，降低血浆肌酸激酶（CK）/乳酸脱氢酶（LDH）活性，减少心肌细胞凋亡。AVLE预处理可明显抑制I/R心肌超氧化物歧化酶（SOD）活性、gp 91（phox）表达和丙二醛（MDA）生成。此外，AVLE治疗增加Akt和细胞外调节蛋白激酶1/2（ERK 1/2）磷酸化在I/R大鼠心脏。磷脂酰肌醇3-激酶（PI 3 K）抑制剂渥曼青霉素或ERK 1/2抑制剂PD 98059阻断AVLE刺激的抗氧化作用和心脏保护作用。我们的研究首次证明，AVLE降低氧化应激，并对大鼠心肌梗死/再灌注损伤发挥心脏保护作用。
Apocynum venetum, a Chinese medicinal herb, is reported to be neuroprotective. However, whether Apocynum venetum leaf extract (AVLE) protects against ischemic myocardium remains elusive. Our present study was aimed to observe the effects of AVLE preconditioning on myocardial ischemia/reperfusion (MI/R) injury and to investigate the possible mechanisms. Rats were treated with AVLE (500 mg/kg/d, o.g.) or distilled water once daily for one week. Afterward, all the animals were subjected to 30 min of myocardial ischemia followed by 4 h of reperfusion. AVLE preconditioning for one week significantly improved cardiac function following MI/R. Meanwhile, AVLE reduced infarct size, plasma creatine kinase (CK)/lactate dehydrogenase (LDH) activities and myocardial apoptosis at the end of reperfusion in rat hearts. Moreover, AVLE preconditioning significantly inhibited superoxide generation, gp91(phox) expression, malonaldialdehyde formation and enhanced superoxide dismutase (SOD) activity in I/R hearts. Furthermore, AVLE treatment increased Akt and extracellular regulated protein kinases 1/2 (ERK1/2) phosphorylations in I/R rat heart. Either the Phosphatidylinositide 3-kinase (PI3K) inhibitor wortmannin or the ERK1/2 inhibitor PD98059 blocked AVLE-stimulated anti-oxidative effects and cardioprotection. Our study demonstrated for the first time that AVLE reduces oxidative stress and exerts cardioprotection against MI/R injury in rats.