Lateral association and elongation of vimentin intermediate filament proteins: A time-resolved light-scattering study
Lateral association and elongation of vimentin intermediate filament proteins: A time-resolved light-scattering study
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DOI：
10.1073/pnas.1606372113
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发表时间：
2016-10-04
影响因子：
11.1
通讯作者：
Koester, Sarah
中科院分区：
文献类型：
--
作者：
Lopez, Carlos G.;Saldanha, Oliva;Koester, Sarah
波形蛋白中间丝（IF）是构成细胞骨架中的三个丝系统之一的蛋白质家族的一部分，是细胞力学的主要贡献者。IF与其他细胞骨架丝类型、肌动蛋白丝和微管的一个区别在于其高度分级的组装途径，其中侧向缔合步骤之后是伸长。在这里，我们提出了一种创新的技术，通过时间分辨的静态和动态光散射跟踪溶液中和原位的延伸反应，从而分别精确地捕获秒到分钟和60-600 nm的相关时间和长度尺度。我们应用定量模型，我们的数据，并成功地一致地描述整个数据集，包括粒子质量，回转半径，流体动力学半径在纵向关联。
Vimentin intermediate filaments (IFs) are part of a family of proteins that constitute one of the three filament systems in the cytoskeleton, a major contributor to cell mechanics. One property that distinguishes IFs from the other cytoskeletal filament types, actin filaments and microtubules, is their highly hierarchical assembly pathway, where a lateral association step is followed by elongation. Here we present an innovative technique to follow the elongation reaction in solution and in situ by time-resolved static and dynamic light scattering, thereby precisely capturing the relevant time and length scales of seconds to minutes and 60-600 nm, respectively. We apply a quantitative model to our data and succeed in consistently describing the entire set of data, including particle mass, radius of gyration, and hydrodynamic radius during longitudinal association.