HUMAN EPIDERMAL T CELLS PREDOMINATELY BELONG TO THE LINEAGE EXPRESSING a / 0 T CELL RECEPTOR
HUMAN EPIDERMAL T CELLS PREDOMINATELY BELONG TO THE LINEAGE EXPRESSING a / 0 T CELL RECEPTOR
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人表皮 T 细胞主要属于表达 a/0 T 细胞受体的谱系
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
K. Wolff
中科院分区：
文献类型：
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作者：
Carolyn A. Foster;H. Yokozeki;Klemens Rappersberger;Frits Koning;B. Volc;Armin Rieger;John E. Coligan;K. Wolff
人类正常的表皮，长期以来被认为仅仅是身体的保护性覆盖物，现在被认为是一个复杂的免疫单位。这一概念是基于朗格汉斯细胞(LCS)是各种T细胞(TC)反应的强大的APC以及角质形成细胞(KC)至少在刺激时可以分泌过量的免疫调节细胞因子(见参考文献1)的证明。最后，近40年来，人们已经知道正常的人类表皮含有淋巴细胞样细胞，但令人惊讶的是，从那时起，这种细胞群几乎没有受到关注。最近在小鼠表皮中发现了含TCRy/S的淋巴细胞群(DECC，树突状表皮T细胞)，重新引起了人们的兴趣，即表皮不仅可能作为胸腺外微环境对TCS的分化和/或“教育”起作用，而且在人类表皮中也可能存在TC群，特别是相当于小鼠的DECC。虽然一些研究人员从未或很少在正常皮肤中观察到表皮TCS(3，4)，但我们和其他研究人员发现，在非皮损、临床正常的皮肤中经常可以检测到它们(5-9)。在这项研究中，我们试图(A)更全面地描述表皮TC的异质性表型，特别是关于携带TCR-a/o和-y/b的亚群，(B)检查免疫标记的表皮TC的超微结构特征，(C)对不同身体区域的这一群体进行定量，(D)检查其在
Normal human epidermis, long since dismissed as merely a protective covering for the body, is now recognized as a complex immunological unit . This concept is based on the demonstration that Langerhans cells (LCs)t are potent APCs for a variety of T cell (TC) responses and that keratinocytes (KC), at least upon stimulation, can secrete a plethora of immunomodulating cytokines (reviewed in reference 1) . Finally, it has been known for almost 40 years that the normal human epidermis harbors lymphocytic-appearing cells (2), yet surprisingly this cell population has received little attention since then . The relatively recent discovery ofaTCRy/S-bearing lymphocyte population (DETC, dendritic epidermal Tcell) in the mouse epidermis (reviewed in reference 1) has rekindled interest in the possibility not only that the epidermis mayfunction as an extrathymic microenvironment for the differentiation and/or "education" of TCs but also that a resident TC population may exist in the human epidermis, particularly an equivalent to the murine DETC. While some investigators never, or only rarely, observed epidermal TCs in normal skin (3, 4), we and others have found them to be routinely detectable in nonlesional, clinically normal skin (5-9). In this study we have sought to (a) more fully describe the heterogeneous phenotype ofepidermal TCs, particularly with regard to TCR-a/o-and --y/b-bearing subpopulations, (b) examine ultrastructural features of immunolabeled epidermal TCs, (c) quantitate this population in various body regions, (d) examine their in