Content of the Growth Factors bFGF, IGF-1, VEGF, and PDGF-BB in Freshly Harvested Lipoaspirate after Centrifugation and Incubation
Content of the Growth Factors bFGF, IGF-1, VEGF, and PDGF-BB in Freshly Harvested Lipoaspirate after Centrifugation and Incubation
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DOI：
10.1097/prs.0b013e318199ef31
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发表时间：
2009-03-01
影响因子：
3.6
通讯作者：
Wolter, Timm P.
中科院分区：
文献类型：
--
作者：
Pallua, Norbert;Pulsfort, Ann K.;Wolter, Timm P.
背景：在自体脂肪移植中，关于移植物基本生化特性的若干问题仍未得到解答。碱性成纤维细胞生长因子（bFGF）、胰岛素样生长因子（IGF）、血管内皮生长因子（VEGF）以及血小板源性生长因子（PDGF）-BB已被证明可改善移植效果。然而，关于移植脂肪组织中生长因子自然存在水平的研究尚未开展。此外，术中提纯是否会消除或浓缩生长因子也尚无相关数据。
方法：作者通过酶联免疫吸附测定读数，研究了新鲜抽吸的脂肪组织中bFGF、IGF、VEGF和PDGF - BB的含量，以及离心后各组分中这些因子的存在情况。他们还分析了储存3天和5天后的生长因子含量，并通过Hoechst 33342和碘化丙啶染色研究了新鲜离心脂肪组织的活力。
结果：作者在用于移植的吸脂物中发现了大量的bFGF、IGF - 1、VEGF和PDGF - BB（分别为39.9、113.8、3.0和5.8皮克/毫克）。通过离心分离并丢弃下层液和上层液意味着大部分生长因子留在了移植物中。其他组分中的生长因子含量明显较低。吸脂物可培养数天，组织仍具有生物活性并产生显著的生长因子水平。
结论：了解这些生长因子数量的自然存在范围将使研究人员和临床医生能够使自体脂肪移植手术更加可靠和安全。（《整形与重建外科》2009年；123卷：826页）
Background: In autologous fat transfer, several questions regarding basic biochemical properties of the transplant remain unanswered. Basic fibroblast growth factor (bFGF), insulin-like growth factor (IGF), vascular endothelial growth factor (VEGF), and platelet-derived growth factor (PDGF)-BB have been shown to improve transplantation results. However, no study regarding naturally occurring levels of growth factors in transplanted adipose tissue has yet been performed. In addition, there are no data as to whether intraoperative refinement eliminates or concentrates growth factors.Methods: The authors investigated the content of bFGF, IGF, VEGF, and PDGF-BB in freshly aspirated adipose tissue and the presence of those factors in the various fractions after centrifugation by means of enzyme-linked immunosorbent assay readings. They also analyzed growth factor content after storage periods of 3 and 5 days and investigated the vitality of freshly centrifuged adipose tissue by staining with Hoechst 33342 and propidium iodide.Results: The authors found significant quantities of bFGF, IGF-1, VEGF, and PDGF-BB (39.9, 113.8, 3.0, and 5.8 pg/mg, respectively) in the lipoaspirate harvested for transplantation. Separation by centrifugation and discarding of the infranatant and supernatant fluid means that most of the growth factors are left in the transplant. The growth factor content in the other fractions was significantly lower. Lipoaspirate can be cultured for several days, with the tissue remaining biologically active and producing significant growth factor levels.Conclusion: Knowledge of the naturally occurring range for these growth factor quantities will enable researchers and clinicians to make autologous fat transfer procedures more reliable and safe. (Plast. Reconstr. Surg. 123: 826, 2009.)