Molecular characterization and genotyping of methicillin-resistant Staphylococcus aureus in nasal carriage of healthy Iranian children
Molecular characterization and genotyping of methicillin-resistant Staphylococcus aureus in nasal carriage of healthy Iranian children
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DOI：
10.1099/jmm.0.000924
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发表时间：
2019-03-01
影响因子：
3
通讯作者：
Rostami, Soodabeh
中科院分区：
文献类型：
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作者：
Mobasherizadeh, Sina;Shojaei, Hasan;Rostami, Soodabeh
目的.社区相关耐甲氧西林金黄色葡萄球菌（CA-MRSA）已成为发达国家和发展中国家的一个相当大的公共卫生问题，由于这种细菌在世界各地的快速传播，MRSA的流行病学也发生了变化，因为MRSA菌株的分离不仅限于医疗机构或具有易感风险因素的患者。因此，本研究的目的是确定伊朗儿童中CA-MRSA鼻携带的遗传多样性和抗生素耐药性。2013年4月至2014年3月进行了一项横断面研究。从410名无危险因素的学龄前儿童的前鼻孔中分离出25株CA-MRSA。通过检测Panton-Valentine杀白细胞素（pvl）和γ-溶血素基因对所有MRSA分离株进行表征。葡萄球菌盒式染色体mec分型（SCCmec）和多位点序列分型（MLST）。在25株CA-MRSA分离株中，分别在1株（4%）和18株（72%）分离株中检测到Pvl和γ-溶血素基因。总体而言，92%（23/25）的分离株属于SCCmec IV型，其中8%（2/25）具有SCCmec V型特征。MLST将25株分离株分为6个克隆复合体（CC）和8个序列型（ST）（CC 5/ST 6、CC 22/ST 22和ST 217、CC 30/ST 30和ST 1107、CC 78/ST 859、CC 398/ST 291和CC 97/ST 405）。ST 859/SCCmec IV（11/25，44%）为优势克隆。ST 859-MRSA-IV-pvl阴性（对四环素耐药）已成功适应伊朗学龄前儿童人群。我们的研究结果表明，CA-MRSA之间的基因组多样性。此外，目前的研究表明，pvl不是我们地区CA-MRSA的可靠标志物。
Purpose. Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has become a considerable public health concern in both developed and developing countries due to the rapid spread of this bacterium around the world, also the epidemiology of MRSA has changed, as the isolation of MRSA strains is not limited to health-care settings or patients with predisposing risk factors. Therefore, the objective of this study is to determine the genetic diversity and antibiotic resistance profile of CA-MRSA nasal carriage in Iranian children.Methodology. A cross-sectional study was conducted from April 2013 to March 2014. A total of 25 CA-MRSA were isolated from the anterior nares of 410 preschool children with no risk factors. All MRSA isolates were characterized by detection of the Panton-Valentine leukocidin (pvl) and gamma-hemolysin genes. staphylococcal cassette chromosome mec (SCCmec) typing and multi-locus sequence typing (MLST).Results. In 25 CA-MRSA isolates, Pvl and gamma-hemolysin genes were detected in one (4%) and 18 (72%) isolates; respectively. Overall, 92% (23/25) of isolates belonged to SCCmec type IV and 8% (2/25) of them had SCCmec type V profile. Using MLST, the 25 isolates were grouped into six clonal complexes (CC) and eight sequence types (ST) (CC5/ST6, CC22/ST22 and ST217, CC30/ST30 and ST1107, CC78/ST859, CC398/ST291 and CC97/ST405). The ST859/SCCmec IV (11/25, 44%) was the predominant clone among the isolates. ST859-MRSA-IV-pvl-negative (resistant to tetracycline) have successfully adapted to the Iranian preschool children population.Conclusion. Our results suggest that the genomic diversity was observed among the CA-MRSA. In addition, the current study demonstrates that pvl is not a reliable marker for CA-MRSA in our region.