The two-component response regulator RcsB regulates type 1 piliation in Escherichia coli.
The two-component response regulator RcsB regulates type 1 piliation in Escherichia coli.
复制标题
双组分反应调节剂 RcsB 调节大肠杆菌中的 1 型毛发起毛作用。
DOI：
10.1128/jb.00705-07
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发表时间：
2007
影响因子：
3.2
通讯作者：
Wolfe,AlanJ
中科院分区：
文献类型：
--
作者：
Schwan,WilliamR;Shibata,Satoshi;Aizawa,Shin-Ichi;Wolfe,AlanJ
大肠杆菌细胞产生1型皮利的能力取决于fimA启动子的方向。取向取决于FimB和FimE重组酶的比率。在此，我们报道了双组分反应调节剂RcsB通过控制fimB和fimE转录影响纤毛状态。1型皮利是由肠杆菌科的许多成员产生的丝状蛋白质附属物（4），在人类感染过程中的生物膜发育和发病机制中起主要作用（26）。大肠杆菌细胞可以从完全有纤毛的状态转变为完全无纤毛的状态（7）。这种能力取决于一个称为相位变化的过程（图1），其中一个314 bp的可逆DNA元件在两个可相互转换的方向之间切换（1）。当该元件处于“相开”方向时，fimA启动子（fimAp）面对fim操纵子，因此可以驱动其转录。由于fim操纵子包括菌毛组装所需的结构成分和机制的基因，处于“阶段”方向的细胞精心制作了许多1型皮利。当该元件处于“相位关闭”状态时，启动子朝向相反的方向，启动子不能驱动fimA转录，并且细胞完全缺乏1型皮利。fimAp元件的可逆性质取决于两种位点特异性重组酶FimB和FimE。而FimE重组酶活性有利于从“相开”到“相关”的切换，FimB促进在两个方向上的切换（16，22，23）。
The ability of Escherichia coli cells to produce type 1 pili depends upon the orientation of the fimA promoter. The orientation depends upon the ratios of the FimB and FimE recombinases. Here, we report that the two-component response regulator RcsB influences the piliation state by controlling fimB and fimE transcription.Type 1 pili are filamentous proteinaceous appendages produced by many members of the family Enterobacteriaceae (4) that play a major role in biofilm development and pathogenesis during the course of human infections (26). Escherichia coli cells can switch from a completely piliated state to a completely nonpiliated state (7). This ability depends on a process called phase variation (Fig. 1), in which a 314-bp invertible DNA element switches between two interconvertible orientations (1). When this element is in the “phase-on” orientation, the fimA promoter (fimAp) faces the fim operon and thus can drive its transcription. Since the fim operon includes genes for structural components and the machinery required for pilus assembly, cells in the “phase-on” orientation elaborate numerous type 1 pili. When the element is oriented in the “phase-off” state, the promoter faces the opposite direction, the promoter cannot drive fimA transcription, and the cells lack type 1 pili altogether. The invertible nature of the fimAp element depends upon two site-specific recombinases, FimB and FimE. Whereas FimE recombinase activity favors switching from “phase-on” to “phase-off,” FimB facilitates switching in both directions (16, 22, 23).