Determination of pyrimidine cyclobutane dimers by electrospray ionization/ion trap mass spectrometry
Determination of pyrimidine cyclobutane dimers by electrospray ionization/ion trap mass spectrometry
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电喷雾电离/离子阱质谱法测定嘧啶环丁烷二聚体
DOI：
10.1002/bms.1200210705
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发表时间：
1992
影响因子：
2.3
通讯作者：
G. Glish
中科院分区：
文献类型：
--
作者：
R. Ramsey;G. J. Berkel;S. A. McLuckey;G. Glish
介绍了一种基于电喷雾电离/离子陷阱质谱法测定环丁烷嘧啶二聚体、胸腺嘧啶-胸腺嘧啶、尿嘧啶-胸腺嘧啶和尿嘧啶-尿嘧啶的直接方法。这些化合物被检测为盐化分子[M+Na]+，通过在电喷雾溶液中添加低浓度的钠盐来最大化对其的响应。在此条件下，通过流动注射分析测定二聚体的亚纳克级。结构信息是通过碰撞诱导锂加合物[M+Li]+解离获得的，这些加合物是由锂盐添加到溶剂中形成的。
A straightforward approach for determining the cyclobutane pyrimidine dimers, thymine-thymine, uracil-thymine, and uracil-uracil, based upon electrospray ionization/ion trap mass spectrometry is described. These compounds are detected as the sodiated molecules, [M + Na]+, the response for which is maximized by adding low concentrations of sodium salts to the electrospray solutions. Under these conditions subnanogram levels of the dimers are determined by flow injection analysis. Structural information is obtained by collision-induced dissociation of the lithium adducts, [M + Li]+, which are formed by adding lithium salts to the solvents.