Dextran sulfate sodium (DSS) colitis in rats - Clinical, structural, and ultrastructural aspects
Dextran sulfate sodium (DSS) colitis in rats - Clinical, structural, and ultrastructural aspects
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DOI：
10.1023/a:1026620322859
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发表时间：
1999-07-01
影响因子：
3.1
通讯作者：
Caprilli, R
中科院分区：
文献类型：
--
作者：
Gaudio, E;Taddei, G;Caprilli, R
本研究的目的是评估结构，超微结构，免疫组织化学和临床方面的Sprague-Dawley大鼠与葡聚糖硫酸钠（DSS）诱导的结肠炎。在Sprague-Dawley大鼠中通过7天DSS口服给药，随后7天仅自来水给药（1、2和3个周期）诱导结肠炎。对照组仅喂以水。充分制备每只动物的近端、中段和远端结肠节段，用于光学和扫描电子显微镜观察。对病变的严重程度进行组织学评分。对于免疫组织化学研究，使用S-100、NSE和抗神经丝抗体的混合物。每日记录体重、大便粘稠度、腹泻、便血等症状。从临床角度看，第1周期后症状出现时间明显晚于第2、3周期，第2、3周期持续时间明显延长。与对照组相比，治疗组大鼠的体重增加率降低了20%，并且与对照组相比，结肠炎诱导后整个结肠长度似乎显著缩短。光学显微镜结构观察显示远端结肠明显受累。粘膜下和肌间神经丛的免疫组织化学研究与对照组相似。结肠炎大鼠结肠粘膜表面的扫描电子显微镜观察显示其结构完全颠覆，其特征是腺体隐窝开口扩张、杯状细胞脱落以及微绒毛分布不均匀或缺乏，这些在第三个周期后最为明显。总之，SD大鼠的实验性DSS结肠炎似乎具有高度可重复性，并且不仅从结构和临床角度，而且从超微结构角度来看，与人类UC具有大多数特征。
Aim of this study was to assess the structural, ultrastructural, immunohistochemical, and clinical aspects in Sprague-Dawley rats with dextrane sulfate sodium (DSS)-induced colitis. Colitis was induced in Sprague-Dawley rats by seven days of DSS oral administration followed by seven days of tap water only (for one, two and three cycles). Controls were fed with water only. Segments of proximal, mid-, and distal colon of each animal were adequately prepared for light and scanning electron microscope observations. The severity of the lesions was scored histologically, For immunohistochemical study, a cocktail of S-100, NSE, and anti-neurofilament antibodies was used. Symptoms such as weight, feces consistency, diarrhea, hematochezia were recorded daily, From a clinical point of view symptoms appeared significantly later after the first cycle than after the second and third cycles and lasted significantly longer in the second and third cycles. Treated rats showed a slower weight gain rate by 20% compared to controls, and the whole colon length appeared to be significantly shorter after colitis induction compared to controls. Structural observations by light microscopy showed prominent involvement of the distal colon. Immunohistochemical study of both submucosal and myoenteric nerve plexuses was similar to controls. Scanning electron microscope observations of the colonic mucosal surface in colitis rats showed a complete subversion of its architecture, characterized by dilatations of gland crypt openings, dropout of goblet cells, and inhomogeneous distribution or lack of microvilli, These were most evident after the third cycle. In conclusion, experimental DSS colitis in SD rats appeared to be highly reproducible and shared most features with human UC, not only from a structural and clinical but also from an ultrastructural point of view.