Quantitatively determined uptake of cell-penetrating peptides in non-mammalian cells with an evaluation of degradation and antimicrobial effects
Quantitatively determined uptake of cell-penetrating peptides in non-mammalian cells with an evaluation of degradation and antimicrobial effects
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DOI：
10.1016/j.peptides.2006.01.006
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发表时间：
2006-07-01
期刊：
影响因子：
3
通讯作者：
Hallbrink, Mattias
中科院分区：
文献类型：
--
作者：
Palm, Caroline;Netzerea, Semharai;Hallbrink, Mattias
细胞穿透肽 (CPP) 是为提高哺乳动物细胞对反义寡核苷酸和短干扰 RNA 等重要研究工具的吸收而开发的载体。然而，非哺乳动物细胞摄取 CPP 的数据有限。我们使用荧光高效液相色谱法，在昆虫细胞 (Sfg)、革兰氏阳性菌 (巨大芽孢杆菌)、革兰氏阴性菌 (大肠杆菌) 和酵母 (酿酒酵母) 四种常见模型系统中，研究了三种代表性肽 penetratin（源自非哺乳动物蛋白）、MAP（人工肽）和 pVEC（源自哺乳动物蛋白）的摄取和抗菌效果。我们证明非哺乳动物细胞内化 CPP，并且对肽摄取的比较表明，肽的细胞内浓度和降解在生物体之间存在很大差异。此外，这些 CPP 还表现出抗菌活性。 (c) 2006 Elsevier Inc. 保留所有权利。
Cell-penetrating peptides (CPPs) are carriers developed to improve mammalian cell uptake of important research tools such as antisense oligonucleotides and short interfering RNAs. However, the data on CPP uptake into non-mammalian cells are limited. We have studied the uptake and antimicrobial effects of the three representative peptides penetratin (derived from a non-mammalian protein), MAP (artificial peptide) and pVEC (derived from a mammalian protein) using fluorescence HPLC in four common model systems: insect cells (Sfg), gram-positive bacteria (Bacillus megaterium), gram-negative bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae). We demonstrate that non-mammalian cells internalize CPPs and a comparison of the uptake of the peptides show that the intracellular concentration and degradation of the peptides varies widely among organisms. In addition, these CPPs showed antimicrobial activity. (c) 2006 Elsevier Inc. All rights reserved.