Large-scale protein structure modeling of the Saccharomyces cerevisiae genome
Large-scale protein structure modeling of the Saccharomyces cerevisiae genome
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DOI：
10.1073/pnas.95.23.13597
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发表时间：
1998-11-10
影响因子：
11.1
通讯作者：
Sali, A
中科院分区：
文献类型：
--
作者：
Sánchez, R;Sali, A
蛋白质的功能通常由其三维 (3D) 结构决定。因此，了解从许多基因组计划中出现的数千个蛋白质序列的 3D 结构将很有用。为此，折叠分配、比较蛋白质结构建模和模型评估完全自动化。作为说明，该方法应用于酿酒酵母（面包酵母）基因组中的蛋白质。它产生了 1,071 种（17%）酵母蛋白的全原子 3D 模型，其中只有 40 种通过实验确定了其 3D 结构。在过去建模的 1,071 种蛋白质中，有 236 种首次与已知结构的蛋白质明确相关；其中 41 个以前根本没有被表征过。
The function of a protein generally is determined by its three-dimensional (3D) structure, Thus, it would be useful to know the 3D structure of the thousands of protein sequences that are emerging from the many genome projects. To this end, fold assignment, comparative protein structure modeling, and model evaluation were automated completely. As an illustration, the method was applied to the proteins in the Saccharomyces cerevisiae (baker's yeast) genome. It resulted in all-atom 3D models far substantial segments of 1,071 (17%) of the yeast proteins, only 40 of which have had their 3D structure determined experimentally, Of the 1,071 modeled past proteins, 236 were related clearly to a protein of known structure for the first time; 41 of these previously have not been characterized at all.