Rootstock-induced scion resistance against tobacco mosaic virus is associated with the induction of defence-related transcripts and graft-transmissible mRNAs.
Rootstock-induced scion resistance against tobacco mosaic virus is associated with the induction of defence-related transcripts and graft-transmissible mRNAs.
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DOI：
10.1111/mpp.13353
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发表时间：
2023-09
影响因子：
4.9
通讯作者：
Culver, James N.
中科院分区：
文献类型：
--
作者：
Kappagantu, Madhu;Brandon, Matthew;Tamukong, Yvette B.;Culver, James N.
嫁接是一种常见的园艺实践，用于在砧木和接穗之间赋予理想的性状，包括抗病性。为了研究移植物对病毒病害的抗性，将本氏烟草接穗嫁接到不同的番茄砧木上，建立了一种新的异源嫁接系统。benthamiana通常对烟草花叶病毒（TMV）感染高度敏感。然而，发现特定的番茄砧木品种对接种了TMV的benthamiana接穗具有一定的抗性水平。赋予的抗性与病毒积累的延迟和病毒传播的减少有关。RNA测序分析显示，在嫁接到诱导抗性的番茄砧木上的N. benthamiana接穗中，与抗病和植物胁迫相关的转录物富集。利用抗性和非抗性砧木的基因组测序，鉴定了benthamiana接穗内的可移动番茄转录本。与嫁接到非抗性诱导砧木上的类似接穗相比，在抗性诱导的benthamiana接穗中，富集的活动番茄转录本主要与防御、胁迫和脱落酸信号相关。综合这些发现表明，嫁接诱导的抗性是由砧木接穗转录反应和砧木特异性移动转录调节的。本研究比较了对烟草花叶病毒敏感或抗性的番茄/本烟异种嫁接系统，以鉴定砧木诱导的接穗反应和移动mRNA相关嫁接诱导的抗性。
Grafting is a common horticultural practice used to confer desirable traits between rootstock and scion, including disease resistance. To investigate graft‐conferred resistance against viral diseases a novel heterografting system was developed using Nicotiana benthamiana scions grafted onto different tomato rootstocks. N. benthamiana is normally highly susceptible to tobacco mosaic virus (TMV) infection. However, specific tomato rootstock varieties were found to confer a range of resistance levels to N. benthamiana scions inoculated with TMV. Conferred resistance was associated with delays in virus accumulation and the reduction in virus spread. RNA sequencing analysis showed the enrichment of transcripts associated with disease resistance and plant stress in N. benthamiana scions grafted onto resistance‐inducing tomato rootstocks. Genome sequencing of resistance‐ and nonresistance‐conferring rootstocks was used to identify mobile tomato transcripts within N. benthamiana scions. Within resistance‐induced N. benthamiana scions, enriched mobile tomato transcripts were predominantly associated with defence, stress, and abscisic acid signalling when compared to similar scions grafted onto nonresistance‐inducing rootstock. Combining these findings suggests that graft‐induced resistance is modulated by rootstock scion transcriptional responses and rootstock‐specific mobile transcripts. Tomato/Nicotiana benthamiana heterograft systems displaying either susceptibility or resistance to tobacco mosaic virus were compared to identify rootstock‐induced scion responses and mobile mRNA‐associated graft‐induced resistance.
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影响因子：
4.6
作者：
González R;Butković A;Rivarez MPS;Elena SF
通讯作者：
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影响因子：
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DOI：
10.1002/9780470650851.ch2
发表时间：
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Horticultural Reviews
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