Effects of implantation site of stem cell grafts on behavioral recovery from stroke damage
Effects of implantation site of stem cell grafts on behavioral recovery from stroke damage
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DOI：
10.1161/01.str.0000027693.50675.c5
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发表时间：
2002-09-01
期刊：
影响因子：
8.3
通讯作者：
Hodges, H
中科院分区：
文献类型：
--
作者：
Modo, M;Stroemer, P;Hodges, H
背景和目的-将MHP36干细胞移植到大脑中动脉闭塞引起的病变对侧的完整实质中促进中风缺陷的恢复，这促使我们研究干细胞的植入部位是否影响MHP36移植物的功能功效方法-将MHP36细胞(200 000/8μL)植入左侧(n=8)或右侧(n=9)实质或输注到右侧实质60 分钟腔内右侧大脑中动脉闭塞诱发中风后 2 至 3 周，在脑室（脑室内 n=7）中另外对完整对照组（n=11）和中风对照组（n=7）进行为期 14 周的双侧不对称旋转偏差和空间学习任务测试，然后对细胞分布和分化进行组织学研究。脑室内移植物，但仍然存在明显的不对称性 相对于中风对照，没有移植组表现出安非他明诱导的旋转偏差减少或病变体积减少 在所有移植组中，细胞占据大脑两侧 纹状体中移植的细胞的三分之一穿过中线，占据完整和病变半球的同源区域，并分化成适当位置的表型 结论 - 中风后，完整和病变半球都吸引移植的干细胞，表明修复过程可以利用然而，实质和心室内移植物的不同作用表明，中风引起的认知和感觉运动缺陷的恢复涉及不同的机制。
Background and Purpose-Findings that MHP36 stem cells grafted into intact parenchyma contralateral to the lesion induced by middle cerebral artery occlusion promoted recovery from stroke deficits led us to investigate whether implantation site of stem cells affects the functional efficacy of MHP36 graftsMethods-MHP36 cells (200 000/8 muL) were implanted in the left (n=8) or right (n=9) parenchyma or infused into the right ventricle (intraventricular n=7) 2 to 3 weeks after stroke induced by 60 minutes of intraluminal right middle cerebral artery occlusion Additionally intact (n=11) and stroke (n=7) control groups were tested for 14 weeks in bilateral asymmetry rotation bias and spatial learning tasks before histological investigation of cell distribution and differentiationResults-Rats with left and right parenchymal grafts showed reduced bilateral asymmetry but no improvement in spatial learning Conversely spatial learning improved in rats with intraventricular grafts but marked asymmetry persisted No grafted group showed reduced amphetamine induced rotation bias or reduced lesion volume relative to stroke controls In all grafted groups cells occupied both sides of the brain A third of cells grafted in the striatum crossed the midline to occupy homologous regions in intact and lesioned hemispheres and differentiated into site appropriate phenotypesConclusions-After stroke both the intact and lesioned hemispheres attract grafted stem cells suggesting repair processes that utilize cells both for local repair and to augment plastic changes, in contralateral motor pathways However differential effects of parenchymal and intraventricular grafts suggest that different mechanisms are implicated in recovery from cognitive and sensorimotor deficits induced by stroke.