Growth of human normal and leukemic T cells: T-cell growth factor (TCGF) and the isolation of a new class of RNA tumor viruses (HTLV).
Growth of human normal and leukemic T cells: T-cell growth factor (TCGF) and the isolation of a new class of RNA tumor viruses (HTLV).
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人类正常和白血病 T 细胞的生长：T 细胞生长因子 (TCGF) 和一类新型 RNA 肿瘤病毒 (HTLV) 的分离。
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发表时间：
1981
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影响因子：
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通讯作者：
Gallo Rc
中科院分区：
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作者：
Gallo Rc
本实验室于5年前建立了液体悬浮培养人成熟T淋巴细胞的常规长期培养体系。这个系统包括连续使用一种称为T细胞生长因子(TCGF)的因子来增殖先前由凝集素(PHA或ConA)或抗原激活的T淋巴细胞。正常的人类T细胞不会对TCGF产生反应，除非它们首先被抗原或凝集素激活成为原始细胞，这可能是因为它们在激活之前不包含TCGF受体。因此，我们认为TCGF是T淋巴细胞免疫反应中的生理性生长促进剂，作为免疫反应中的第二信号(仅次于抗原)。这些T细胞表现出功能特征，例如，细胞毒性或辅助功能以及其他成熟的证据。通过使用不含PHA和TCGF以外的淋巴因子的纯化TCGF，我们最近已经能够从白血病和成熟T细胞淋巴瘤患者中培养出常规肿瘤性T细胞，例如来自Sezary综合征和真菌样肉芽肿(皮肤T细胞白血病和淋巴瘤)的患者。值得注意的是，肿瘤T细胞直接对TCGF产生反应；与正常T细胞不同，它们不需要在体外预先用抗原或凝集素激活。这表明转化的成熟T细胞已经表达TCGF受体。这可能是正常和转化的人类T细胞之间的一个重要的功能差异。我们最近从这些新的细胞系中分离出了一类新的RNA肿瘤病毒(逆转录病毒)，我们称之为HTLV。出于几个原因，我们认为这是第一批明确的人类RNA肿瘤病毒分离株。例如，我们现在有数据表明，这些新病毒很容易与所有以前从动物身上分离的病毒区分开来。此外，我们在一些患有这些疾病的患者的血清中发现了与这些病毒的蛋白质特异反应的抗体。
: A system for routine long-term growth of human mature T-lymphocytes in liquid suspension culture was developed in our laboratory about 5 years ago. This system involves the continuous use of a factor, termed T-cell growth factor (TCGF) for the propagation of T-lymphocytes previously activated by lectin (PHA or Con A) or by antigen. Normal human T cells do not respond to TCGF unless they are first activated to become blast cells by antigen or lectin, presumably because they do not contain TCGF receptors until activated. We think then that TCGF is the physiological growth promoter in the immune response of T-lymphocytes, acting as the second signal (after antigen) in the immune response. These T cells show functional features, e.g., cytotoxic or helper function and other evidence of maturity. By using purified TCGF free of PHA and of lymphokines other than TCGF, we have recently been able to grow routinely neoplastic T cells from patients with leukemias and lymphomas of mature T cells, e.g., from patients with the Sezary syndrome and those with mycosis fungoides (cutaneous T-cell leukemias and lymphomas). Of considerable interest, the neoplastic T cells respond directly to TCGF; unlike normal T Cells they do not require prior in vitro activation with antigen or lectin. This indicates that transformed mature T cells already express TCGF receptors. This may be an important functional difference between normal and transformed human T cells. From some of these new cell lines we have recently isolated a new class of RNA tumor viruses (retroviruses), which we call HTLV. For several reasons we believe these are the first unambiguous isolates of RNA tumor viruses from humans. For instances, we now have data that these new viruses are easily distinguishable from all previously isolated viruses from animals. In addition, we have found antibodies in sera of some patients with these diseases specifically reactive with proteins of these viruses.