Distribution of the different capsular types of Streptococcus suis in nineteen swine nurseries.
Distribution of the different capsular types of Streptococcus suis in nineteen swine nurseries.
复制标题
十九个猪舍中猪链球菌不同荚膜类型的分布。
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
M. Gottschalk
中科院分区：
文献类型：
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作者：
J. L. Flores;R. Higgins;S. D’Allaire;R. Charette;M. Boudreau;M. Gottschalk
猪链球菌血清型2是最常见的从病猪分离的包膜类型，其次是包膜类型3、1/2、8和4(1)。猪链球菌囊型2型在临床健康动物中的流行率在不同的畜群中从0%到90%不等。在加拿大，4-8周龄临床健康仔猪(3，4)和707头屠宰的猪(5)的上呼吸道中有2%至4%的囊膜2型存在。在临床健康的猪中，囊型17、18、19和21型代表大多数猪链球菌分离株(6)。由于最近已经描述了新的包膜类型[7]，所以29种包膜类型的猪链球菌在临床健康猪中的流行率尚未确定。本研究的目的是确定目前已知的所有断奶仔猪的包膜类型的流行率。从19个饲养场抽取了958头4-8周龄的仔猪，这些托儿所为同一产业组织拥有的8个不同的育肥工序提供饲喂猪。这些托儿所的平均库存为240头仔猪，平均每群随机选择50头动物。在收集标本时，所有分娩单位和托儿所都没有临床症状，并且在过去6个月中没有经历过任何与猪链球菌有关的疾病问题。出于实际原因，鼻拭子比扁桃体拭子更受欢迎。动物被束缚在一个坐着的位置。用浸泡在酒精中的无菌纱布清洁一个外鼻孔，并用无菌拭子(Culturette，Ingram和Bell Science，Ville St-Laurent，魁北克)在8至10厘米深(4)处收集样本。拭子在4摄氏度下保存，并于当天进行实验室程序。血液琼脂平板(5%牛血)含有选择性补充剂SR-126(加拿大，内皮安，安大略省)用于培养拭子，并在37℃孵育24小时，根据不同类型的菌落数量，从每个平板中选择最多6个α-溶血菌落。每个菌落划线到血液琼脂平板上进行继代培养。在37℃下孵育24小时后，将培养物加工并在70℃下保持，直到鉴定时。
Streptococcus suis serotype 2 is the capsular type most frequently isolated from diseased pigs, followed, in decreasing order, by capsular types 3, 1/2, 8, and 4 (1). The prevalence of S. suis capsular type 2 in clinically healthy animals varies from 0% to 90% among herds (2). In Canada, capsular type 2 was shown to be present in the upper respiratory tract of 2% to 4% of four to eight-week-old clinically healthy piglets (3,4) and of 707o of pigs at slaughter (5). In clinically healthy pigs, capsular types 17, 18, 19, and 21 represented the majority of S. suis isolates (6). Since new capsular types have been described recently (7), the prevalence of each of the 29 capsular types of S. suis in clinically healthy pigs has not yet been established. The aim of the present study was to determine the prevalence of all presently known capsular types in weaned piglets. A total of 958 four to eight-week-old piglets was sampled from 19 nurseries that provided feeder pigs for eight different finishing operations owned by the same industrial organization. The mean inventory in these nurseries was 240 piglets, and an average of 50 animals was randomly selected in each of the herds. At the time of collecting the specimens, all farrowing units and nurseries were free of clinical signs, and had not experienced any disease problem associated with S. suis in the previous six months. Nasal swabs were preferred to tonsilar swabs for practical reasons. Animals were restrained in a seated position. One external naris was cleaned with a sterile gauze soaked in alcohol and samples were collected with a sterile swab (Culturette, Ingram and Bell Scientific, Ville St-Laurent, Quebec) twisted at a depth of 8 to 10 cm (4). Swabs were held at 4°C, and laboratory procedures were undertaken the same day. Blood agar plates (5% bovine blood) containing the selective supplement SR-126 (Oxoid Canada, Nepean, Ontario) were used for the culture of swabs and were incubated at 37°C for 24 h. Depending on the number of different types of colonies, a maximum of six alpha-hemolytic colonies were selected from each plate. Each colony was streaked onto a blood agar plate for subculture. After 24 h of incubation at 37°C, cultures were processed and held at 70°C until the time of identification.