Expression and Regulation of Insulin and the Glucose Transporter GLUT8 in the Testes of Diabetic Rats
Expression and Regulation of Insulin and the Glucose Transporter GLUT8 in the Testes of Diabetic Rats
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DOI：
10.1055/s-0028-1128146
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发表时间：
2009-05-01
影响因子：
2.2
通讯作者：
Terrado, J.
中科院分区：
文献类型：
--
作者：
Gomez, O.;Ballester, B.;Terrado, J.
糖尿病会导致男性生殖细胞出现多种功能障碍。本研究的目的是分析单剂量链脲佐菌素诱导糖尿病状态的大鼠睾丸中葡萄糖转运蛋白 GLUT8 和胰岛素的水平和定位。诱导糖尿病1个月后，糖尿病大鼠的GLUT8免疫反应性主要位于精细胞顶体系统，在Leydig细胞中处于低水平。与对照大鼠相比，该转运蛋白的免疫组织化学定位及其水平均未显示任何差异。此外，观察到对照大鼠睾丸表达胰岛素，其广泛位于间质细胞和早期细长精子细胞的细胞质中，并集中在更成熟精子细胞的细胞质区室中。通过蛋白质印迹法测得的糖尿病大鼠睾丸胰岛素水平降低了一半以上，但激素的分布没有变化。这些结果表明，i) 胰岛素由睾丸细胞产生，ii) 链脲佐菌素诱导的糖尿病会消耗胰岛素，iii) 胰岛素消耗和高血糖不会调节睾丸中 GLUT8 的表达。这些结果还表明，睾丸产生的胰岛素可以在调节这些器官中的精子发生和/或葡萄糖代谢中发挥作用。
Diabetes induces several malfunctions in male germ cells. The aim of this Study was to analyze the levels and localization of the glucose transporter GLUT8 and insulin in the testes of rats induced to a diabetic status by a single dose of streptozotocin. One month after inducing diabetes, the GLUT8 immunoreactivity in diabetic rats was mainly located associated to the acrosomic system of spermatids, and at low levels in Leydig cells. Neither the immumohistochemical localization of this transporter nor its levels showed any difference when compared to control rats. Furthermore, it was observed that control rat testes expressed insulin, which was diffusely located in the cytoplasm of both Leydig cells and early elongated spermatids and concentrated in a cytoplasmic compartment in the more mature spematids. Testicular insulin levels measured by western blot were reduced by more than half in diabetic rats, although the distribution of the hormone was unchanged. These results indicate that i) insulin is produced by testicular cells, ii) insulin is depleted by streptozotocin-induced diabetes, and iii) that insulin depletion and hyperglycemia do not regulate the expression of GLUT8 in testes. These results also Suggest that testicular production Of insulin Could play a role in regulating spematogenesis and/or glucose metabolism in these organs.