The Arabidopsis SDG4 contributes to the regulation of pollen tube growth by methylation of histone H3 lysines 4 and 36 in mature pollen
The Arabidopsis SDG4 contributes to the regulation of pollen tube growth by methylation of histone H3 lysines 4 and 36 in mature pollen
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DOI：
10.1016/j.ydbio.2007.12.016
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发表时间：
2008-03-15
影响因子：
2.7
通讯作者：
Fukui, Kiich
中科院分区：
文献类型：
--
作者：
Cartagena, Joyce A.;Matsunaga, Sachihiro;Fukui, Kiich
植物SET结构域蛋白参与植物发育过程中基因表达的表观遗传调控。本文报道拟南芥SET结构域蛋白SDG4参与花粉管生长的表观遗传调控，从而影响受精。利用SDG4- gfp融合构建了SDG4在烟草BY-2细胞中的染色体定位。在拟南芥中，sdg4敲除显示出生殖缺陷。组织特异性表达分析表明，SDG4是花粉中主要表达的ash1相关基因。免疫学分析表明，SDG4参与了花序和花粉粒中历史H3的甲基化。sdg4花粉营养核中历史H3 K4和K36甲基化量的显著减少导致花粉管生长受到抑制。我们的研究结果表明，SDG4能够通过甲基化营养细胞核中组蛋白H3的特定赖氨酸残基来调节花粉管生长中功能基因的表达。(C) 2007爱思唯尔公司版权所有。
Plant SET domain proteins are known to be involved in the epigenetic control of gene expression during plant development. Here, we report that the Arabidopsis SET domain protein, SDG4, contributes to the epigenetic regulation of pollen tube growth, thus affecting fertilization. Using an SDG4-GFP fusion construct, the chromosomal localization of SDG4 was established in tobacco BY-2 cells. In Arabidopsis, sdg4 knockout showed reproductive defects. Tissue-specific expression analyses indicated that SDG4 is the major ASH1-related gene expressed in the pollen. Immunological analyses demonstrated that SDG4 was involved in the methylation of historic H3 in the inflorescence and pollen grains. The significant reduction in the amount of methylated historic H3 K4 and K36 in sdg4 pollen vegetative nuclei resulted in suppression of pollen tube growth. Our results indicate that SDG4 is capable of modulating the expression of genes that function in the growth of pollen tube by methylation of specific lysine residues of the histone H3 in the vegetative nuclei. (C) 2007 Elsevier Inc. All rights reserved.