IL-31 is associated with cutaneous lymphocyte antigen-positive skin homing T cells in patients with atopic dermatitis
IL-31 is associated with cutaneous lymphocyte antigen-positive skin homing T cells in patients with atopic dermatitis
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DOI：
10.1016/j.jaci.2005.10.046
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发表时间：
2006-02-01
影响因子：
14.2
通讯作者：
Gross, JA
中科院分区：
文献类型：
--
作者：
Bilsborough, J;Leung, DYM;Gross, JA
背景资料：IL-31是一种新发现的T细胞细胞因子，当其在小鼠中过度表达时，会导致类似于人类特应性皮炎（AD）的瘙痒和皮肤皮炎。目的：方法：采用免疫组化和RT-PCR方法检测AD患者和健康人皮肤活检组织和外周血细胞中IL-31和IL-31 RA的表达。皮肤归巢皮肤淋巴细胞抗原（CLA）阳性T细胞的IL-31蛋白的生产也assessed.Results：IL-31 RA蛋白表达的角质形成细胞和浸润的巨噬细胞在皮肤活检标本AD患者。AD患者皮肤与健康皮肤的比较显示，AD样品中表皮角质形成细胞上的IL-31 RA表达水平较高。与健康个体相比，AD患者皮肤中的浸润细胞更多，表达IL 31 mRNA。这些细胞的组织形态计量学分析表明，它们是淋巴细胞谱系，大多数细胞对CLA和CD 3染色呈阳性。AD患者和健康志愿者外周血中IL 31 mRNA和蛋白表达主要局限于CD 45 RO（+）（记忆）CLA（+）T细胞。此外，与来自健康个体的CLA（+）T细胞相比，来自AD患者而不是来自银屑病患者的循环CLA（+）T细胞能够产生更高水平的IL-31。结论：IL-31的表达与CLA（+）T细胞相关，可能参与AD诱导的皮肤炎症和瘙痒的发生。
Background: IL-31 is a newly discovered T-cell cytokine that, when overexpressed in mice, results in pruritus and skin dermatitis resembling human atopic dermatitis (AD). Objective: We sought to investigate the expression of IL-31 and IL-31 receptor A (IL-31RA) in skin biopsy specimens and peripheral blood cells from patients with AD and healthy individuals.Methods: Expression of IL-31 and IL-31RA was evaluated in skin biopsy specimens from patients with AD and healthy individuals by means of immunohistochemistry and RT-PCR. IL-31 protein production by skin-homing cutaneous lymphocyte antigen (CLA)-positive T cells was also assessed.Results: IL-31RA protein was expressed by keratinocytes and infiltrating macrophages in skin biopsy specimens from patients with AD. Comparisons between skin from patients with AD and healthy skin showed IL-31RA expression at higher levels on epidermal keratinocytes in AD samples. Infiltrating cells, more numerous in skin from patients with AD compared with that of healthy individuals, expressed IL31 mRNA. Histomorphometric analysis of these cells indicated they were of the lymphocytic lineage, with the majority of cells staining positive for CLA and CD3. IL31 mRNA and protein expression is largely restricted to CD45RO(+) (memory) CLA(+) T cells in peripheral blood of patients with AD and healthy volunteers. Moreover, circulating CLA(+) T cells from patients with AD, but not from patients with psoriasis, are capable of producing I higher levels of IL-31 compared with CLA(+) T cells from healthy individuals. However, the average levels of IL-31 were not significantly different between patients with AD and healthy individuals.Conclusion: We provide evidence that IL-31 expression is associated with CLA(+) T cells and might contribute to the development of AD-induced skin inflammation and pruritus.