Characterization of signal sequences determining the nuclear export of Newcastle disease virus matrix protein
Characterization of signal sequences determining the nuclear export of Newcastle disease virus matrix protein
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DOI：
10.1007/s00705-013-1769-5
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发表时间：
2013-12-01
影响因子：
2.7
通讯作者：
Liu, Xiufan
中科院分区：
文献类型：
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作者：
Duan, Zhiqiang;Song, Qingqing;Liu, Xiufan
新城疫病毒（NDV）基质(M)蛋白已被证明是一种核细胞质转运蛋白。先前的研究表明，M蛋白是通过核定位信号在细胞核中定位的。在这里，我们报道了M蛋白穿梭到细胞质的能力是由三个核输出信号序列（NESs）介导的。使用leptomycin B (LMB)，一种特异性的CRM1抑制剂，我们发现三个NESs的核输出是LMB不敏感的，因此是CRM1独立的。此外，这些NESs的失活导致M蛋白的核积累。我们的研究结果强调了这些NESs对NDV M蛋白核输出的重要性。
The Newcastle disease virus (NDV) matrix (M) protein has been demonstrated to be a nuclear-cytoplasmic trafficking protein. Previous studies have shown that the M protein localizes in the nucleus through a bipartite nuclear localization signal. Here, we report that the ability of the M protein to shuttle to the cytoplasm is mediated by three nuclear export signal sequences (NESs). Using leptomycin B (LMB), a specific inhibitor of CRM1, we found that the nuclear export of the three NESs was LMB insensitive and thus was CRM1 independent. In addition, inactivation of these NESs led to nuclear accumulation of the M protein. Our results highlight the significance of these NESs to the nuclear export of the NDV M protein.