Polymer Monoliths with Exchangeable Chemistries: Use of Gold Nanoparticles As Intermediate Ligands for Capillary Columns with Varying Surface Functionalities
Polymer Monoliths with Exchangeable Chemistries: Use of Gold Nanoparticles As Intermediate Ligands for Capillary Columns with Varying Surface Functionalities
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DOI：
10.1021/ac1015613
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发表时间：
2010-09-01
影响因子：
7.4
通讯作者：
Frechet, Jean M. J.
中科院分区：
文献类型：
--
作者：
Cao, Qing;Xu, Yan;Frechet, Jean M. J.
新开发的多孔聚合物整体毛细管柱采用涂有可交换功能的金纳米颗粒进行改性，可通过简单的配体交换过程轻松切换分离模式。这些柱由聚(甲基丙烯酸缩水甘油酯-共聚-亚乙基二甲基丙烯酸酯)整料通过其环氧化物部分与半胱胺反应制备，以提供富含表面硫醇基团的整料。通过在柱内原位还原氯金酸制备的金纳米颗粒附着在整料孔隙的表面。或者，可以将胶体金纳米颗粒溶液泵入硫醇改性柱以实现其附着。虽然第一种方法速度更快，但与第二种方法相比，它提供的纳米颗粒覆盖率较低，而两种方法都保留了整体柱典型的优异流体动力学特性。然后使用低分子量含硫醇表面配体对结合的金纳米颗粒的表面进行官能化。金和这些表面配体之间键的动态性质使得能够通过简单的溶液交换过程用另一种表面配体替换一种表面配体。这种新颖的方法扩大了整体柱的应用范围，因为单个柱现在可以用于不同的分离模式。具有可交换化学物质的色谱柱的应用通过肽的毛细管电色谱分离和反相和离子交换模式下的蛋白质纳米高压液相色谱 (HPLC) 分离得到了证明。
Newly developed porous polymer monolithic capillary columns modified with gold nanoparticles coated with exchangeable functionalities allow easy switching of separation modes by a simple ligand exchange process. These columns are prepared from a poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith through reaction of its epoxide moieties with cysteamine to afford a monolith rich in surface thiol groups. Gold nanoparticles prepared via in situ reduction of chloroauric acid within the column become attached to the surface of the pores of the monolith. Alternatively, a solution of colloidal gold nanoparticles can be pumped through the thiol modified column to achieve their attachment. While the first approach is faster, it affords a lower coverage of nanoparticles than the second method, while both methods preserve the excellent hydrodynamic properties that are typical of the monolithic columns. Functionalization of the surface of the bound gold nanoparticles is then carried out using low molecular weight thiol-containing surface ligands. The dynamic nature of the bond between gold and these surface ligands enables the replacement of one surface ligand by another through a simple solution exchange process. This novel approach expands the application range of monoliths as a single column can now be used in different separations modes. Applications of the columns with exchangeable chemistries are demonstrated with the capillary electrochromatographic separation of peptides and the nano-high-pressure liquid chromatography (HPLC) separation of proteins in both reversed phase and ion exchange modes.