Subterranean infestation by Holotrichia parallela larvae is associated with changes in the peanut (Arachis hypogaea L.) rhizosphere microbiome
Subterranean infestation by Holotrichia parallela larvae is associated with changes in the peanut (Arachis hypogaea L.) rhizosphere microbiome
复制标题
平行黑鳃金龟幼虫的地下侵染与花生 (Arachishypogaea L.) 根际微生物组的变化有关
DOI：
10.1016/j.micres.2018.02.008
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发表时间：
2018-01-01
影响因子：
6.7
通讯作者：
Zhang, Jie
中科院分区：
文献类型：
--
作者：
Geng, Li-Li;Shao, Gao-Xiang;Zhang, Jie
根际微生物有助于作物的健康和发育，当植物经历生物/非生物胁迫时，这些有益的微生物被招募到根区。花生地下害虫Holotricia parallela可造成严重的作物损失。菲尔兹。通过对花生16S rRNA基因V3和V4高变区的深度测序，分析了侵染和未侵染花生根际细菌的特征，认为侵染花生幼虫可能影响根际微生物群落组成。总共产生了2,673,656个读数，平均每个样本获得了2558个OTU。通过对花生根际细菌群落结构的比较，发现花生根际中变形杆菌和拟杆菌的相对丰度增加，而放线菌的相对丰度降低。通过主坐标分析发现，在感染后24 h内，细菌群落发生了明显的变化。在处理24 h内鉴定出的332个属中，有67个属的丰度发生了显著变化。实时荧光定量聚合酶链式反应证实感染样本中的假单胞菌属在24小时内有所增加。我们的研究结果表明，花生幼虫侵染能迅速引起花生根际微生物群落的变化和特定细菌种类的丰富。但这种影响并不是持久的。这项研究为深入了解根际微生物在地下害虫与作物相互作用中的作用提供了依据。
Rhizosphere microorganisms contribute to the health and development of crops and these beneficial microbes are recruited to the root-zone when plants experience biotic/abiotic stress. The subterranean pests Holotrichia parallela cause severe crop loss in peanut (Arachis hypogaea L.) fields. Hypothesizing that infestation by H. parallela larva may influence the composition of rhizosphere microbial communities, deep sequencing of V3 and V4 hypervariable regions of 16S rRNA gene was used to characterize the rhizosphere bacteria of infested and uninfested peanuts. A total of 2,673,656 reads were generated and an average of 2558 OTUs were obtained for each sample. Comparisons of rhizosphere bacterial community structure of peanuts with those infested by H. parallela larva revealed that the relative abundance of Proteobacteria and Bacteroidetes increased, while that of Actinobacteria decreased in the rhizosphere with infestation. A significant shift in bacterial communities was observed within 24 h after infestation by principal coordinate analysis. For the 332 genera identified in 24 h treatment, infestation of white grubs led to the significant changes of abundance of 67 genera. An increase in the Pseudomonas genus of infested-samples for 24 h was verified by real-time qPCR. Our results indicate H. parallela larvae infestation can quickly leads to the change of peanut rhizosphere microbiome and enrichment of specific bacterial species. But the effects were not persistent. This study provides the insight into the function of rhizosphere microbiome in the interaction between subterranean pests and crops.