Physical, functional and conditional interactions between ArcAB and phage shock proteins upon secretin-induced stress in Escherichia coli
Physical, functional and conditional interactions between ArcAB and phage shock proteins upon secretin-induced stress in Escherichia coli
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DOI：
10.1111/j.1365-2958.2009.06809.x
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发表时间：
2009-10-01
影响因子：
3.6
通讯作者：
Buck, Martin
中科院分区：
文献类型：
--
作者：
Jovanovic, Goran;Engl, Christoph;Buck, Martin
在肠杆菌中发现的噬菌体休克蛋白（Psp）系统是对受损的内膜完整性的反应（其中Psp反应被认为有助于维持细胞的质子动力），并且与病原体如耶尔森氏菌和沙门氏菌的毒力有关。我们提供的证据表明，双组分ArcAB系统参与诱导大肠杆菌中的Psp反应，现在报告说，ArcAB的作用是有条件的。ArcAB主要通过ArcA调节基因的作用，但也通过直接的ArcB-Psp相互作用，需要在微需氧过程中传播蛋白IV（pIV）依赖性psp诱导信号，但在需氧或厌氧过程中不需要。我们表明，ArcB直接与PspB相互作用，可能是通过PspB亮氨酸拉链基序，从而允许两个系统之间的交叉通信。此外，我们表明，pIV依赖的诱导psp的表达在厌氧是独立的PspBC，建立PspA和PspF可以作为一个最小的Psp系统响应内膜应力。
P>The phage shock protein (Psp) system found in enterobacteria is induced in response to impaired inner membrane integrity (where the Psp response is thought to help maintain the proton motive force of the cell) and is implicated in the virulence of pathogens such as Yersinia and Salmonella. We provided evidence that the two-component ArcAB system was involved in induction of the Psp response in Escherichia coli and now report that role of ArcAB is conditional. ArcAB, predominantly through the action of ArcA regulated genes, but also via a direct ArcB-Psp interaction, is required to propagate the protein IV (pIV)-dependent psp-inducing signal(s) during microaerobiosis, but not during aerobiosis or anaerobiosis. We show that ArcB directly interacts with the PspB, possibly by means of the PspB leucine zipper motif, thereby allowing cross-communication between the two systems. In addition we demonstrate that the pIV-dependent induction of psp expression in anaerobiosis is independent of PspBC, establishing that PspA and PspF can function as a minimal Psp system responsive to inner membrane stress.