Oridonin enhances the radiosensitivity of lung cancer cells by upregulating Bax and downregulating Bcl-2
Oridonin enhances the radiosensitivity of lung cancer cells by upregulating Bax and downregulating Bcl-2
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DOI：
10.3892/etm.2018.6803
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发表时间：
2018-12-01
影响因子：
2.7
通讯作者：
Cheng, Guanghui
中科院分区：
文献类型：
--
作者：
Li, Sirui;Shi, Dan;Cheng, Guanghui
冬凌草甲素是中药冬凌草的有效成分。本研究旨在评价冬凌草甲素对人非小细胞肺癌（NSCLC）细胞的抗肿瘤作用，并探讨冬凌草甲素是否能增强NSCLC细胞的放射敏感性。采用MTT法检测冬凌草甲素对肺癌细胞SPC-A-1和HCC 827的增殖抑制作用，并呈时间和剂量依赖性。另外，照射前给予冬凌草甲素预处理24 h可提高SPC-A-1细胞的放射敏感性。Western blotting检测细胞凋亡调节因子Bax和Bcl-2的表达。结果表明，与单纯照射组相比，冬凌草甲素与照射联合作用可使SPC-A-1细胞Bax表达增加，Bcl-2表达降低。这些结果表明，冬凌草甲素可能有一个新的应用作为一种放射增敏剂治疗人类非小细胞肺癌。
Oridonin is an active component of the traditional Chinese herb Rabdosia rubescens. The present study aimed to evaluate the antitumor effects of oridonin on human non-small cell lung cancer (NSCLC) cells and explore whether oridonin could enhance their radiosensitivity. Oridonin was demonstrated to inhibit the proliferation of SPC-A-1 and HCC827 lung cancer cells in a time- and dose-dependent manner, which was detected using the MTT assay. In addition, pretreatment with oridonin for 24 h prior to irradiation was identified to enhance the radiosensitivity of SPC-A-1 cells. Furthermore, the levels of apoptosis regulator BAX (Bax) and apoptosis regulator Bcl-2 (Bcl-2) were detected by western blotting analysis. The results demonstrated that the level of Bax was increased and the level of Bcl-2 was decreased in SPC-A-1 cells treated with oridonin and irradiation compared with the group that received irradiation alone. These results indicate that oridonin may have a novel application as a radiosensitizing agent for the treatment of human NSCLC.