SYSTEMIC VIRULENCE OF ERWINIA-CHRYSANTHEMI 3937 REQUIRES A FUNCTIONAL IRON ASSIMILATION SYSTEM
SYSTEMIC VIRULENCE OF ERWINIA-CHRYSANTHEMI 3937 REQUIRES A FUNCTIONAL IRON ASSIMILATION SYSTEM
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DOI：
10.1128/jb.170.6.2419-2426.1988
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发表时间：
1988-06-01
影响因子：
3.2
通讯作者：
EXPERT, D
中科院分区：
文献类型：
--
作者：
ENARD, C;DIOLEZ, A;EXPERT, D
在欧文氏菌，铁饥饿的条件下开始生产的儿茶酚型铁载体，并提高生产的三个外膜多肽。22个突变体在不同阶段的铁同化系统的影响，分离的mini-Mu插入诱变。所有这些都未能诱导无菌生长的圣保罗植物上的系统性软腐病。从铁载体营养缺陷型和铁摄取突变体中，通过使用体内克隆载体pULB 113（RP 4：：mini-Mu）分离具有恢复缺失功能的克隆。一个含有ca.鉴定出35.5-内切酶对DNA插入片段。铁功能的恢复部分恢复，如果不是完全恢复，亲本菌株的毒力。
In Erwinia chrysanthemi, conditions of iron starvation initiate production of a catechol-type siderophore and enhance production of three outer membrane polypeptides. Twenty-two mutants affected in the different stages of this iron assimilation system were isolated by mini-Mu insertion mutagenesis. All of them failed to induce systemic soft rot on axenically grown Saintpaulia plants. From the siderophore auxotrophs and the iron uptake mutants, clones having recovered the missing function(s) were isolated by using the in vivo cloning vector pULB113 (RP4::mini-Mu). An R-prime plasmid containing a ca. 35.5-kilobase-pair DNA insert was identified. Restoration of the iron functions restored partially, if not completely, the virulence of the parental strain.