Peptidomic Analysis of Female Reproductive Tract Secretion to Identify Putative Anti-Infection Peptides in the Female Genital System via Nanotechnologies
Peptidomic Analysis of Female Reproductive Tract Secretion to Identify Putative Anti-Infection Peptides in the Female Genital System via Nanotechnologies
复制标题
通过纳米技术对女性生殖道分泌物进行肽组学分析，鉴定女性生殖系统中假定的抗感染肽
DOI：
10.1166/jbn.2018.2500
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发表时间：
2018
影响因子：
2.9
通讯作者：
Zeng Xin
中科院分区：
文献类型：
--
作者：
Yan Lina;Luan Ting;Hua Quan;Gu Yun;Fu Ziyi;Liu Xia;Li Minghui;Liu Chengxin;Li Ping;Zeng Xin
女性生殖系统感染是各种妇科疾病中的高发人群。关于妇科感染，内源性肽在女性生殖道分泌中的作用很少被研究。为了鉴定与内源性肽相关的妇科感染，本研究使用纳流液相色谱-串联质谱仪对性成熟和围绝经期女性的阴道分泌物进行了比较肽组学分析。共鉴定出源自1364个前体蛋白的3096个肽片段，其中102个存在差异表达，其中与围绝经期组相比，性成熟组中有60个过表达肽和42个表达不足的肽。 6种差异表达的生物活性肽被鉴定与免疫缺陷病毒（HIV）感染、人乳头瘤病毒（HPV）感染、细菌性阴道病（BV）和外阴阴道念珠菌病（VVC）相关。鉴定出源自粘蛋白家族的 MUC4、MUC5A、MUC5B、MUC6、MUC17 和 MUC19 肽可增强阴道粘液抵抗感染的免疫力。此外，随着生物医学纳米技术的显着发展，我们研究中发现的潜在抗感染肽将有助于妇科感染的诊断和治疗，特别是与纳米颗粒结合使用时。总之，我们的研究结果将填补女性生殖系统感染肽组学的空白，并为未来生殖道疾病管理的研究铺平道路。
The female genital system infections have high-incidence among various gynecological diseases. The role of endogenous peptides in female reproductive tract secretion has been rarely investigated regarding gynecological infections. To identify gynecological infections associated endogenous peptides, a comparative peptidomic profiling of vaginal secretion from sexually mature and perimenopause women was conducted in this study using nanoflow liquid chromatography-tandem mass spectrometer. A total of 3096 peptide fragments originating from 1364 precursor proteins were identified, 102 of which were discrepantly expressed, which included 60 over-expressed peptides and 42 under-expressed peptides in the sexually mature group compared with the perimenopause group. 6 differentially expressed bioactive peptides were identified to be related to immunodeficiency virus (HIV) infections, human papillomavirus (HPV) infections, bacterial vaginosis (BV) and vulvovaginal candidiasis (VVC). MUC4, MUC5A, MUC5B, MUC6, MUC17 and MUC19 peptides originating from mucin family were identified to enhance vaginal mucous immunity against infections. Moreover, with remarkable development of biomedical nanotechnology, the identified potential anti-infection peptides in our study will be useful in diagnosis and treatment of gynecological infections, especially when combined with nanoparticles. In conclusion, our findings will fill the gap on peptidomics for female genital system infections and pave the way for future studies about reproductive tract diseases management.