Platelet-derived growth factor and malignant transformation.
Platelet-derived growth factor and malignant transformation.
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血小板源性生长因子和恶性转化。
DOI：
10.1016/0006-2952(84)90202-8
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发表时间：
1984
影响因子：
5.8
通讯作者：
Antoniades,HN
中科院分区：
文献类型：
--
作者：
Antoniades,HN
对人血小板衍生生长因子（PDGF）（培养物中的间充质衍生细胞的有效有丝分裂原[1 -3]）的研究为理解恶性转化中涉及的至少一种机制提供了合理的基础。PDGF是一种热稳定的（100”）阳离子（等电点9.8）多肽[4]，在血小板α颗粒中储存的血液中循环[5]。在血液凝固过程中从血小板释放到血清中，构成血清的主要多肽生长因子。我们最近发现，血小板源性生长因子（PDGF）与猴肉瘤病毒（SSV）的转化蛋白共同参与创伤愈合过程。一种灵长类起源的急性转化逆转录病毒，来源于相同或密切相关的细胞基因[6]。该结论基于以下证明：PDGF和SSV转化蛋白[7，8]具有广泛的氨基酸序列同源性16，9，101，具有共同的抗原决定簇和结构构象[111，并发挥相同的生物学功能[12，131。这些发现表明，猴肉瘤病毒诱导转化的能力来自于逆转录病毒基因组中PDGF基因的整合。逆转录病毒基因组内的转化一基因（v-sis）区域编码PDGF样有丝分裂原，并能够通过持续产生这种引起持续细胞增殖的有效有丝分裂原诱导肿瘤转化。
Investigations of human platelet-derived growth factor (PDGF), a potent mitogen for mesenchymal derived cells in culture [l-3], have provided a rational basis for the understanding of at least one mechanism involved in malignant transformation. PDGF is a heat-stable(loo”), cationic (isolectric point 9.8) polypeptide[4] that circulates in blood stored in the a-granules of platelets [5]. It is released from platelets into the serum during blood clotting, constituting the major polypeptide growth factor of serum. It is suggested that in uiuo PDGF is delivered during platelet degranulation at the site of injury where it participates in the process of wound healing by stimulating the proliferation and migration of connective tissue cells.We have shown recently that PDGF and the transforming protein of the simian sarcoma virus (SSV). an acute transforming retrovirus of primate origin, derive from the same or closely related cellular genes [6]. This conclusion is based on the demonstration that PDGF and the SSV transforming protein [7, 8] share extensive amino acid sequence homology 16, 9, 101, have common antigenic determinants and structural conformation[111, and exert identical biological functions [12, 131. These findings suggest that the ability of the simian sarcoma virus to induce transformation derives from the incorporation of the PDGF gene within the retroviral genome. The resulting transforming one gene (v-sis) region within the retrovirus genome codes for a PDGF-like mitogen and is capable of inducing neoplastic transformation by the continuous production of this potent mitogen causing sustained cell proliferation.