Paenibacillus glycanilyticus subsp. hiroshimensis subsp. nov., isolated from leaf soil collected in Japan
Paenibacillus glycanilyticus subsp. hiroshimensis subsp. nov., isolated from leaf soil collected in Japan
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DOI：
10.1007/s00203-020-02166-6
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发表时间：
2021-01-22
影响因子：
2.8
通讯作者：
Kimura, Zen-ichiro
中科院分区：
文献类型：
--
作者：
Akita, Hironaga;Itoiri, Yuya;Kimura, Zen-ichiro
菌株CCI 5是从日本采集的叶土中分离到的一株贫营养细菌。菌株CCI 5在25 ° C至43 ° C的温度（最适温度，40 ° C）和6.0至10.0的pH（最适pH，9.0）下生长。其主要脂肪酸为anteiso-C-15：0和iso-C-16：0，甲基萘醌7是唯一检测到的醌系统。16 S rRNA基因序列分析表明，菌株CCI 5属于类芽孢杆菌属（Paenibacillus）。此外，基于atpD、dnaA、gl和infB基因的部分序列的多位点序列分析显示，菌株CCI 5与解聚糖假单胞菌DS-1（T）紧密聚簇。CC 15菌株的基因组草图由6，864，972 bp组成，G+C含量为50.7%，并包含6，189个预测编码序列。菌株CCI 5与P. glycanilyticus DS-1（T）的基因组平均核苷酸同一性值（97.8%）低于原核亚种划分的临界值。基于其表型、化学分类学和系统发育特征，菌株CCI 5（= HUT-8145（T）= KCTC 43270（T））可以被认为是类芽孢杆菌属内的新亚种，其建议名称为解聚糖类芽孢杆菌亚种（Paenibacillusglycanilyticus subsp.）。广志亚种Nov.
Strain CCI5, an oligotrophic bacterium, was isolated from leaf soil collected in Japan. Strain CCI5 grew at temperatures between 25 degrees C and 43 degrees C (optimum temperature, 40 degrees C) and at pHs between 6.0 and 10.0 (optimum pH, 9.0). Its major fatty acids were anteiso-C-15:0 and iso-C-16:0, and menaquinone 7 was the only detected quinone system. In a phylogenetic analysis based on 16S rRNA gene sequences, strain CCI5 presented as a member of the genus Paenibacillus. Moreover, multilocus sequence analysis based on partial sequences of the atpD, dnaA, gmk, and infB genes showed that strain CCI5 tightly clustered with P. glycanilyticus DS-1(T). The draft genome of strain CCI5 consisted of 6,864,972 bp with a G+C content of 50.7% and comprised 6,189 predicted coding sequences. The genome average nucleotide identity value (97.8%) between strain CCI5 and P. glycanilyticus DS-1(T) was below the cut-off value for prokaryotic subspecies delineation. Based on its phenotypic, chemotaxonomic, and phylogenetic features, strain CCI5 (= HUT-8145(T) = KCTC 43270(T)) can be considered as a novel subspecies within the genus Paenibacillus with the proposed name Paenibacillus glycanilyticus subsp. hiroshimensis subsp. nov.