A modification of the epidermal scarification model of herpes simplex virus infection to achieve a reproducible and uniform progression of disease
A modification of the epidermal scarification model of herpes simplex virus infection to achieve a reproducible and uniform progression of disease
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DOI：
10.1016/s0166-0934(02)00160-x
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发表时间：
2002-12-01
影响因子：
3.1
通讯作者：
Rosenthal, KS
中科院分区：
文献类型：
--
作者：
Goel, N;Docherty, JJ;Rosenthal, KS
在单纯疱疹病毒感染的表皮划伤模型中，对用于去除皮肤顶部角化层的方法进行了轻微的修改，结果是一种更容易、更少痛苦、更均匀和可重复的感染方式。小鼠背部脱毛，用26号针刺侧边刮拭，或用砂纸或手持式电动修脚/修指甲仪研磨，去除表层皮肤。然后将病毒涂抹在结痂或擦伤的皮肤上，观察感染后第3天至第10天小鼠的病变发展情况。皮肤被擦伤的小鼠在第3天出现病变，到第5天进展为带状虫形，病变发展的统一模式，而皮肤被针划伤的小鼠在时间过程、症状进展和更大的创伤方面发生了变化。(C) 2002 Elsevier Science B.V.版权所有
A slight modification in the method used to remove the top keratinized layer of skin in the epidermal scarification model of HSV infection results in an easier, less painful, more uniform and reproducible means of infection. The back of mice was depilated and the top skin layer was removed either by scratching with the side of a 26 guage needle, or by abrading with sand paper or a hand held motorized pedicure/manicure instrument. The virus was then applied on the scarified or abraded skin and the mice were observed for lesion development from day 3 to 10 post-infection. A uniform pattern of lesion development in terms of onset of lesions by day 3, progression to zosteriform by day 5 occurred for mice whose skin was abraded whereas variability in the time course, progression of symptoms and greater trauma occurred for mice whose skin was scratched with needle. (C) 2002 Elsevier Science B.V. All rights reserved.