Antagonistic interaction between male-killing and cytoplasmic incompatibility induced byCardiniumandWolbachiain the whitefly,Bemisia tabaci
Antagonistic interaction between male-killing and cytoplasmic incompatibility induced byCardiniumandWolbachiain the whitefly,Bemisia tabaci
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烟粉虱中Cardinium和Wolbachia诱导的雄性杀伤与细胞质不相容性之间的拮抗相互作用
DOI：
10.1111/1744-7917.12793
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发表时间：
2020-08-14
期刊：
影响因子：
4
通讯作者：
Qiu, Bao-Li
中科院分区：
文献类型：
--
作者：
Lv, Ning;Peng, Jing;Qiu, Bao-Li
Cardinium和Wolbachia是节肢动物的母系遗传细菌共生体，可以通过增加受感染雌性的适应性来操纵宿主繁殖。本文报道了卡氏菌和沃尔巴克氏菌共存于烟粉虱Bemisia tabaciasiII 7中时，可引起杀雄和胞质不亲和性（CI）。Cardinium-Wolbachiacoinfection诱导雄性杀伤，并导致亚洲II 7 I（CWH）和I（CWL）系的种内两亲后代中较高的雌性性别比;有趣的是，雄性杀伤诱导随着Cardiniumtiter的增加而增强。此外，单次感染Wolbachi可导致Asia II 7 I（W）系部分CI，并导致生殖力降低、胚胎死亡率升高和雌性性比降低。未感染的Asia II 7 I（U）系比感染Wolbachia的Asia II 7 I（W）系和Cardinium-Wolbachia-coinfected Asia II 7 I（CWH）系具有显著更高的繁殖力、更低的胚胎和若虫死亡率以及更低的CI水平。我们的研究结果表明，Cardinium-Wolbachiacoinfection诱导雄性杀死，这可能对Wolbachia诱导的CI在亚洲II 7粉虱中具有拮抗作用。我们的研究首次揭示了B. tabaciAsia II 7的繁殖是由Cardinium和Wolbachia内共生体共同操纵的。
CardiniumandWolbachiaare maternally inherited bacterial symbionts of arthropods that can manipulate host reproduction by increasing the fitness of infected females. Here, we report thatCardiniumandWolbachiacoinfection induced male-killing and cytoplasmic incompatibility (CI) when they coexisted in a cryptic species of whitefly,Bemisia tabaciAsia II7.CardiniumandWolbachiasymbionts were either singly or simultaneously localized in the bacteriocytes placed in the abdomen ofB. tabacinymphs and adults.Cardinium-Wolbachiacoinfection induced male-killing and resulted in a higher female sex ratio in the intraspecific amphigenetic progeny of Asia II7 I(CWH)and I(CWL)lines; interestingly, male-killing induction was enhanced with increasedCardiniumtiter. Moreover, single infection ofWolbachiainduced partial CI in the Asia II7 I(W)line and resulted in reduced fecundity, higher embryonic mortality, and lower female sex ratio. The uninfected Asia II7 I(U)line had significantly higher fecundity, lower embryonic and nymphal mortalities, and a lower level of CI than both theWolbachia-infected Asia II7 I(W)line and theCardinium-Wolbachia-coinfected Asia II7 I(CWH)line. Our findings indicate thatCardinium-Wolbachiacoinfection induced male-killing, which may have had antagonistic effects onWolbachia-induced CI in the Asia II7 whiteflies. For the first time, our study revealed thatB. tabaciAsia II7 reproduction is co-manipulated byCardiniumandWolbachiaendosymbionts.