FLUORESCENCE AND OPTICAL CHARACTERISTICS OF REDUCED FLAVINES AND FLAVOPROTEINS
FLUORESCENCE AND OPTICAL CHARACTERISTICS OF REDUCED FLAVINES AND FLAVOPROTEINS
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DOI：
10.1021/bi00700a029
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发表时间：
1974-01-01
期刊：
影响因子：
2.9
通讯作者：
MAYHEW, SG
中科院分区：
文献类型：
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作者：
GHISLA, S;MASSEY, V;MAYHEW, SG
Sandro Ghisla, Vincent Massey,** Jean-Marc Lhoste, and Stephen G. MayhewJ摘要：测量了一系列还原的黄蛋白的荧光和吸收特性，并与合适的模型化合物的特性进行了比较。与通常的看法相反，已经发现许多还原的黄素蛋白在500-530 nm范围内表现出明显的荧光发射。与一般观察结果一致，还原后的黄酮类化合物在室温溶液中没有荧光，但在刚性玻璃中，在77 K下，在476 ~ 512 nm范围内有明显的荧光发射。还原乳酸氧化酶的荧光量子产率（发射Xmax 507 nm）在共价n5加合物构象上提高了4.7倍，最大发射位移到476 nm。在不成功的情况下
Sandro Ghisla, Vincent Massey,** Jean-Marc Lhoste, and Stephen G. MayhewJ abstract: The fluorescence and absorption properties of a series of reduced flavoproteins have been measured and com-pared with the properties of suitable model compounds. Contrary to common belief, a number of reduced flavopro-teins have been found to exhibit appreciable fluorescence emission with maxima in the range 500-530 nm. In keeping with common observation, the reduced model flavines are devoid of fluorescence in solution at room temperature, but show marked fluorescence emission in the range 476-512 nm at 77 K in rigid glasses. The fluorescence quantum yield of re-duced lactate oxidase (emission Xmax 507 nm) is increased 4.7 times uponformation of covalent N 5 adducts and the emission maximum is shifted to 476 nm. In the case of the nonfiuores-