EVIDENCE FOR 2 GENES SPECIFICALLY INVOLVED IN UNSATURATED FATTY ACID BIOSYNTHESIS IN ESCHERICHIA COLI
EVIDENCE FOR 2 GENES SPECIFICALLY INVOLVED IN UNSATURATED FATTY ACID BIOSYNTHESIS IN ESCHERICHIA COLI
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DOI：
10.1128/jb.100.2.601-604.1969
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发表时间：
1969-01-01
影响因子：
3.2
通讯作者：
VAGELOS, PR
中科院分区：
文献类型：
--
作者：
CRONAN, JE;BIRGE, CH;VAGELOS, PR
通过抽提物互补和基于败育转导的遗传互补，将不饱和脂肪酸营养缺陷型大肠杆菌分为两个不同的顺反子。一个顺反子中的损伤导致β-羟基癸酰基硫酯脱氢酶的损失，该酶在不饱和脂肪酸形成中产生第一个生物合成中间体。证据表明，在第二个顺反子的病变导致缺乏第二种酶，特别是参与不饱和脂肪酸的生物合成。
Unsaturated fatty acid auxotrophs ofEschericha colihave been divided into two distinct cistrons by extract complementation and genetic complementation based on abortive transduction. Lesions in one cistron result in the loss of the β-hydroxydecanoyl thioester dehydrase which produces the first biosynthetic intermediate in unsaturated fatty acid formation. Evidence is presented which indicates that lesions in the second cistron result in the lack of a second enzyme specifically involved in the biosynthesis of unsaturated fatty acids.