Dissecting conformational contributions to glycosidase catalysis and inhibition.
Dissecting conformational contributions to glycosidase catalysis and inhibition.
复制标题
DOI：
10.1016/j.sbi.2014.06.003
复制
发表时间：
2014-10
影响因子：
6.8
通讯作者：
Williams, Spencer J.
中科院分区：
文献类型：
--
作者：
Speciale, Gaetano;Thompson, Andrew J.;Davies, Gideon J.;Williams, Spencer J.
构象路线描述了催化过程中糖的形状变化。讨论了糖苷水解的立体电子学要求。主要和新兴的方法来定义构象行程进行审查。总结了糖苷酶构象行程的新归属。糖苷水解酶（GH）被分为>100个基于序列的家族。这些酶加工各种各样的复杂碳水化合物，在异头和其他环位置具有不同的立体化学。这些糖在与它们的同源GH结合时所采用的形状，以及沿催化反应坐标沿着发生的构象变化被称为构象行程。定义GH构象路线的努力集中在反应的临界点上：底物结合（米氏），过渡态，中间体（如果相关）和产物结合。最近的方法来定义构象路线结婚的X射线晶体学的酶结合到配体，模仿的临界点，沿着先进的计算方法和动力学同位素效应进行了讨论。
The conformational itinerary describes the changes in sugar shape during catalysis. Stereoelectronic requirements for glycoside hydrolysis are discussed. Major and emerging approaches to define conformational itineraries are reviewed. New assignments of glycosidase conformational itineraries are summarized. Glycoside hydrolases (GHs) are classified into >100 sequence-based families. These enzymes process a wide variety of complex carbohydrates with varying stereochemistry at the anomeric and other ring positions. The shapes that these sugars adopt upon binding to their cognate GHs, and the conformational changes that occur along the catalysis reaction coordinate is termed the conformational itinerary. Efforts to define the conformational itineraries of GHs have focussed upon the critical points of the reaction: substrate-bound (Michaelis), transition state, intermediate (if relevant) and product-bound. Recent approaches to defining conformational itineraries that marry X-ray crystallography of enzymes bound to ligands that mimic the critical points, along with advanced computational methods and kinetic isotope effects are discussed.
登录
查看更多内容
影响因子：
56.9
作者：
Kim, Jin-Hyo;Resende, Ricardo;Withers, Stephen G.
通讯作者：
Withers, Stephen G.
影响因子：
4.8
作者：
Gregg, Katie J.;Zandberg, Wesley F.;Boraston, Alisdair B.
通讯作者：
Boraston, Alisdair B.
影响因子：
15
作者：
Chan, Jefferson;Lu, April;Bennet, Andrew J.
通讯作者：
Bennet, Andrew J.
DOI：
10.1111/j.1432-1033.1979.tb04219.x
发表时间：
1979-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
LEGLER, G;ROESER, KR;ILLIG, HK
通讯作者：
ILLIG, HK
影响因子：
15
作者：
Mayes, Heather B.;Broadbelt, Linda J.;Beckham, Gregg T.
通讯作者：
Beckham, Gregg T.