Isolation of CD34+ cells from blood stem cell components using the Baxter Isolex system.
Isolation of CD34+ cells from blood stem cell components using the Baxter Isolex system.
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使用 Baxter Isolex 系统从血液干细胞成分中分离 CD34+ 细胞。
DOI：
10.1038/sj.bmt.1701257
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Bensinger,WI
中科院分区：
文献类型：
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作者：
Rowley,SD;Loken,M;Radich,J;Kunkle,LA;Mills,BJ;Gooley,T;Holmberg,L;McSweeney,P;Beach,K;MacLeod,B;Appelbaum,F;Bensinger,WI
CD34抗原由人类造血祖细胞和干细胞表达。这些细胞在去除骨髓的化疗放射治疗后能够重建骨髓功能。已经开发了几种不同的技术来从骨髓或外周血干细胞(PBSC)成分中分离CD34+细胞。我们使用免疫磁分离技术从PBSC成分中富集CD34+细胞，以期为B淋巴系恶性肿瘤患者的自体移植做准备。29名患者参加了这项研究，他们接受了动员化疗，随后接受了G-CSF。其中21例达到了干细胞成分分离方案要求的外周血CD34+细胞水平至少2.0×104/L。收集每个患者三个成分的中位数进行处理。分离后组分中CD34+细胞平均浓度为1.0±1.2%。经CD34+细胞筛选后，富集组分占起始有核细胞的0.6±0.6%。CD34+细胞回收率为起始细胞数的58.4±19.2%，纯度为90.8±6.5%。CD3 4−细胞总耗尽率为99.96±0.0 6%。19例患者接受骨髓刻蚀处理，平均每千克体重获得6.2±2.0×106个CD34+细胞。中位时间11天(8~14天)恢复至ANC和GT 0.5×109/L，中位9天(5~13天)恢复血小板输注。4名患者在移植后100天前死于移植相关并发症或复发。没有患者因为持续的骨髓功能衰竭而需要输注未分离的细胞。这些数据表明，通过免疫磁分离技术浓缩的外周血源CD34+细胞能够在自体移植后快速植入。
The CD34 antigen is expressed by human hematopoietic progenitor and stem cells. These cells are capable of reconstituting marrow function after marrow-ablative chemo-radiotherapy. Several different technologies have been developed for the separation of CD34+ cells from bone marrow or peripheral blood stem cell (PBSC) components. We used an immunomagnetic separation technique to enrich CD34+ cells from PBSC components in anticipation of autologous transplantation for patients with B lymphoid malignancies. Twenty-nine patients enrolled on this study and received mobilization chemotherapy followed by G-CSF. Of these, 21 achieved a peripheral blood CD34+ cell level of at least 2.0× 10 4/l required by protocol for separation of the stem cell components. A median of three components per patient was collected for processing. The average CD34+ cell concentration in the components after apheresis was 1.0±1.2%. After the CD34+ cell selection, the enriched components contained 0.6±0.6% of the starting nucleated cells. The recovery of CD34+ cells, however, averaged 58.4±19.2% of the starting cell number, with a purity of 90.8±6.5%. Overall depletion of CD34− cells was 99.96±0.06%. Nineteen patients were treated with marrow-ablative conditioning regimens and received an average of 6.2±2.0× 10 6 CD34+ cells/kg body weight. These patients recovered to an ANC> 0.5× 10 9/l at a median of 11 days (range 8–14), and platelet transfusion independence at a median of 9 days (range 5–13). Four patients died of transplant-related complications or relapse before 100 days after transplantation. No patient required infusion of unseparated cells because of failure of sustained bone marrow function. These data demonstrate that peripheral blood-derived CD34+ cells enriched by use of an immunomagnetic separation technique are capable of rapid engraftment after autologous transplantation.