Isolation and structure of a novel charged member of the red-pigment-concentrating hormone-adipokinetic hormone family of peptides isolated from the corpora cardiaca of the blowfly Phormia terraenovae (Diptera).
Isolation and structure of a novel charged member of the red-pigment-concentrating hormone-adipokinetic hormone family of peptides isolated from the corpora cardiaca of the blowfly Phormia terraenovae (Diptera).
复制标题
从绿头蝇 Phormia terraenovae（双翅目）心脏体中分离出的红色素浓缩激素-脂肪运动激素肽家族的新型带电成员的分离和结构。
DOI：
10.1042/bj2690309
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Roland KELLNERt
中科院分区：
文献类型：
--
作者：
G. Gáde;Hans WILPSt;Roland KELLNERt
用反相h.p.l.c.方法从绿蝇Phormia terraenoptera的心体中分离出一种高海藻酸盐血症神经肽，并在酶解封闭N-末端焦谷氨酸残基后，采用Edman化学通过脉冲液相测序确定其一级结构。C-末端也被封闭，如肽与羧肽酶A孵育时缺乏消化所示。该八肽具有序列pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH 2，并且被明确定义为RPCH/AKH（红色素浓缩激素/脂肪运动激素）肽家族的新成员。这种合成肽能以剂量依赖的方式增加绿头苍蝇血淋巴中的碳水化合物浓度，因此被命名为“Phormia terraenopathy hypertrehalosaemic hormone”（Pht-HrTH）。此外，美洲大蠊（Periplaneta americana）脂肪体中的受体识别肽，导致血液中碳水化合物的升高。然而，飞蝗（Locusta migratoria）的脂肪体受体不识别这种带电分子，因此在该物种中没有观察到脂质动员。
A hypertrehalosaemic neuropeptide from the corpora cardiaca of the blowfly Phormia terraenovae has been isolated by reversed-phase h.p.l.c., and its primary structure was determined by pulsed-liquid phase sequencing employing Edman chemistry after enzymically deblocking the N-terminal pyroglutamate residue. The C-terminus was also blocked, as indicated by the lack of digestion when the peptide was incubated with carboxypeptidase A. The octapeptide has the sequence pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2 and is clearly defined as a novel member of the RPCH/AKH (red-pigment-concentrating hormone/adipokinetic hormone) family of peptides. It is the first charged member of this family to be found. The synthetic peptide causes an increase in the haemolymph carbohydrate concentration in a dose-dependent fashion in blowflies and therefore is named 'Phormia terraenovae hypertrehalosaemic hormone' (Pht-HrTH). In addition, receptors in the fat-body of the American cockroach (Periplaneta americana) recognize the peptide, resulting in carbohydrate elevation in the blood. However, fat-body receptors of the migratory locust (Locusta migratoria) do not recognize this charged molecule, and thus no lipid mobilization is observed in this species.