Live visualization of chromatin dynamics with fluorescent TALEs
Live visualization of chromatin dynamics with fluorescent TALEs
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DOI：
10.1038/nsmb.2680
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发表时间：
2013-11-01
影响因子：
16.8
通讯作者：
Torres-Padilla, Maria-Elena
中科院分区：
文献类型：
--
作者：
Miyanari, Yusuke;Ziegler-Birling, Celine;Torres-Padilla, Maria-Elena
细胞核中基因组的时空组织是调控基因组功能的一个新兴的关键角色。核组织动态的实时成像将是揭示基因组结构的功能相关性和机制的突破。在这里，我们使用转录激活因子样效应物（TALE）技术来可视化内源性重复基因组序列。我们建立了TALE介导的基因组可视化（TGV），以标记基因组序列，并在培养的小鼠细胞和活生物体中跟踪核定位和染色质动力学。TGV是高度特异性的，因此允许通过区分单核苷酸多态性（SNP）来对亲本染色体进行差异标记。我们的研究结果提供了一个框架，以解决基因组结构的功能，通过可视化的核动力学在体内。
The spatiotemporal organization of genomes in the nucleus is an emerging key player to regulate genome function. Live imaging of nuclear organization dynamics would be a breakthrough toward uncovering the functional relevance and mechanisms regulating genome architecture. Here, we used transcription activator-like effector (TALE) technology to visualize endogenous repetitive genomic sequences. We established TALE-mediated genome visualization (TGV) to label genomic sequences and follow nuclear positioning and chromatin dynamics in cultured mouse cells and in the living organism. TGV is highly specific, thus allowing differential labeling of parental chromosomes by distinguishing between single-nucleotide polymorphisms (SNPs). Our findings provide a framework to address the function of genome architecture through visualization of nuclear dynamics in vivo.