REGULATION OF INTERLEUKIN-2 GENE ENHANCER ACTIVITY BY THE T-CELL ACCESSORY MOLECULE CD28
REGULATION OF INTERLEUKIN-2 GENE ENHANCER ACTIVITY BY THE T-CELL ACCESSORY MOLECULE CD28
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DOI：
10.1126/science.1846244
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发表时间：
1991-01-18
期刊：
影响因子：
56.9
通讯作者：
WEISS, A
中科院分区：
文献类型：
--
作者：
FRASER, JD;IRVING, BA;WEISS, A
细胞表面分子调节T细胞产生淋巴因子的机制知之甚少。白细胞介素-2（IL-2）的产生可以通过与T细胞抗原受体诱导的信号转导途径不同的信号转导途径来调节。刺激CD 28（一种在大多数人T细胞上表达的分子），诱导蛋白复合物的形成，该蛋白复合物与IL-2基因上不同于先前描述的结合位点的位点结合，并使IL-2增强子活性增加5倍。CD 28-应答复合物在转录起始位点的-164和-154碱基对之间与IL-2基因结合。该元件的序列与其它几种淋巴因子基因的5'侧翼保守区相似。
The mechanism by which cell surface molecules regulate T cell production of lymphokines is poorly understood. Production of interleukin-2 (IL-2) can be regulated by signal transduction pathways distinct from those induced by the T cell antigen receptor. Stimulation of CD28, a molecule expressed on most human T cells, induced the formation of a protein complex that bound to a site on the IL-2 gene distinct from previously described binding sites and increased IL-2 enhancer activity fivefold. The CD28-responsive complex bound to the IL-2 gene between - 164 and - 154 base pairs from the transcription start site. The sequence of this element is similar to regions conserved in the 5' flanking regions of several other lymphokine genes.