Surface hydrophobicity of petroleum hydrocarbon degrading Burkholderia strains and their interactions with NAPLs and surfaces
Surface hydrophobicity of petroleum hydrocarbon degrading Burkholderia strains and their interactions with NAPLs and surfaces
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DOI：
10.1016/j.colsurfb.2010.02.019
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发表时间：
2010-06-15
影响因子：
5.8
通讯作者：
Mukherji, Suparna
中科院分区：
文献类型：
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作者：
Chakraborty, Sinjini;Mukherji, Soumyo;Mukherji, Suparna
细菌细胞表面疏水性（CSH）是影响微生物在非水相液体（NAPLs）上生长和粘附行为的重要因素。在这项工作中，CSH和表面电荷定量的三个石油降解伯克霍尔德菌培养物：脂肪族降解洋葱伯克霍尔德菌（ES 1）和两个菌株的芳香族降解多食伯克霍尔德菌（NG 1和HN 1）的接触角和zeta电位测量的基础上。模型非水相液体（NAPLs）配制使用不同浓度的正十六烷，萘，菲和芘。粘附到不同的疏水性和粘附到正十六烷的玻璃表面上进行了定量和相关的表面的疏水性;响应于模型NAPL用作生长底物的培养物的CSH的变化：和作为生长底物、离子强度和再悬浮溶液的pH的变化的结果的ζ电位的变化。B。cepacia（ES 1）和B.多噬菌体（HN 1）的CSH比B高。多噬菌体（NG 1）。对于每一种文化，CSH被认为是不同的模型NAPL用作生长基质。对玻璃的粘附性随着细菌培养物的CSH的增加和玻璃表面的疏水性的增加而增加。B。与B相比，具有较低负ζ电位的洋葱（ES 1）一致地表现出更大的粘附性。多噬菌体（HN 1）。通过统计分析发现，生长基质、pH值、重悬液及其相互作用等因素对正十六烷的粘附性有显著影响。这些因素影响的zeta电位和坚持正十六烷在不同程度上的三个伯克霍尔德氏菌文化。(C)2010 Elsevier B. V.保留所有权利。
Bacterial cell surface hydrophobicity (CSH) is an important factor governing the growth and adhesion behavior of microorganisms on non-aqueous phase liquids (NAPLs). In this work CSH and surface charge was quantified for three oil degrading Burkholderia cultures: aliphatic degrader Burkholderia cepacia (ES1) and two strains of aromatic degrading Burkholderia multivorans (NG1 and HN1) based on contact angle and zeta potential measurement. Model non-aqueous phase liquids (NAPLs) were formulated using n-hexadecane, naphthalene, phenanthrene and pyrene in varying concentration. Adhesion on to glass surfaces of varying hydrophobicity and adherence to n-hexadecane was quantified and correlated with hydrophobicity of the surface; variation in CSH of the culture in response to model NAPL used as growth substrate: and variation in zeta potential as a result of variation in growth substrate, ionic strength and pH of resuspension solution. B. cepacia (ES1) and B. multivorans (HN1) depicted comparable CSH which was higher than that of B. multivorans (NG1). For each culture, CSH was found to vary with the model NAPL used as growth substrate. Adhesion to glass increased with increase in CSH of the bacterial culture and with increase in hydrophobicity of the glass surface. B. cepacia (ES1) with lower negative zeta potential consistently depicted greater adhesion compared to B. multivorans (HN1). Adherence to n-hexadecane was significantly affected by various other factors, such as, growth substrate, pH, resuspension solution and their interactions as revealed through statistical analysis. These factors affected both the zeta potential and adherence to n-hexadecane to varying degree for the three Burkholderia cultures. (C) 2010 Elsevier B.V. All rights reserved.