Phenolic Compounds and Antioxidant Capacities of 10 Common Edible Flowers from China
Phenolic Compounds and Antioxidant Capacities of 10 Common Edible Flowers from China
复制标题
我国10种常见食用花卉的酚类化合物及其抗氧化能力
DOI：
10.1111/1750-3841.12404
复制
发表时间：
2014-04-01
影响因子：
3.9
通讯作者：
Shen, Canxi
中科院分区：
文献类型：
--
作者：
Xiong, Lina;Yang, Jiajia;Shen, Canxi
采用反相高效液相色谱法测定了10种常见食用花卉中的游离态和结合态酚类化合物的含量。以2，2-二苯基-1-苦基肼清除自由基活性、2，2‘-azino-bis(3-ethylbenzothiazoline-6-sulphonic酸(ABTS)清除活性、氧自由基吸收能力、铁还原抗氧化力和细胞抗氧化活性为指标评价其抗氧化能力。在酚类含量和抗氧化能力方面，游离基比结合态更显著。牡丹和金银花的总酚含量最高，分别为235.5 mg绿原酸当量/g干重和89.38 mg芦丁当量/g干重。鉴定出的主要酚类化合物为没食子酸、绿原酸和芦丁。在DPPH、ABTS和ORAC测定中，牡丹的抗氧化能力最强，分别为1028、2065、990molTrolox当量/g干重，而刺梨的FRAP值最高(2645molFe2+当量/g干重)。在磷酸盐缓冲盐水(PBS)和无PBS洗涤方案中，可溶性酚类化合物的CAA最高，其半数有效剂量(EC50)分别为26.7和153mol/100g干重。总酚含量与抗氧化力呈极显著正相关(R=0.8443~0.9978，P<；0.01)，说明酚类物质是影响其抗氧化活性的主要因素。实际应用研究表明，中国所产10种食用花卉含有丰富的植物化学物质，酚类化合物含量高，抗氧化能力强。这项研究不仅为消费者提供了关于食用花卉健康益处的有用信息，还鼓励研究人员将食用花卉作为植物化学物质的来源。此外，花卉提取物在预防慢性病、促进健康、防止食品氧化等方面具有潜在的食品成瘾性。
The free and bound phenolic compounds in 10 common Chinese edible flowers were investigated using reversed phase high-performance liquid chromatography. Their antioxidant capacities were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical-scavenging activity, oxygen radical absorption capacity (ORAC), ferric reducing antioxidant power (FRAP), and cellular antioxidant activity (CAA). Free factions were more prominent in phenolic content and antioxidant capacity than bound fractions. Paeonia suffruticosa and Flos lonicerae showed the highest total phenolic content (TPC) 235.5 mg chlorogenic acid equivalents/g of dry weight and total flavonoid content 89.38 mg rutin equivalents/g of dry weight. The major phenolic compounds identified were gallic acid, chlorogenic acid, and rutin. P. suffruticosa had the highest antioxidant capacity in the DPPH, ABTS, and ORAC assays, which were 1028, 2065, 990mol Trolox equivalents/g of dry weight, respectively, whereas Rosa chinensis had the highest FRAP value (2645mol Fe2+ equivalents /g of dry weight). The P. suffruticosa soluble phenolics had the highest CAA, with the median effective dose (EC50) 26.7 and 153mol quercetin equivalents/100 g of dry weight in the phosphate buffered saline (PBS) and no PBS wash protocol, respectively. TPC was strongly correlated with antioxidant capacity (R = 0.8443 to 0.9978, P < 0.01), which indicated that phenolics were the major contributors to the antioxidant activity of the selected edible flowers.Practical Application This study indicates that the 10 edible flowers in China are rich sources of phytochemicals, with high levels of phenolic compounds and antioxidant capacity. This study not only provides useful information about the health benefit of edible flowers for consumers, but also encourages researchers to utilize edible flowers as sources of phytochemicals. In addition, the flower extracts are potential to be used as addictive in food to prevent the chronic disease, help the health promotion, and prevent the food oxidization.