Preparation and application of molecularly imprinted polymers for the selective extraction of naringin and genistein from herbal medicines
Preparation and application of molecularly imprinted polymers for the selective extraction of naringin and genistein from herbal medicines
复制标题
分子印迹聚合物的制备及其在中药中选择性提取柚皮苷和染料木黄酮的应用
DOI：
10.1039/c9ay01503e
复制
发表时间：
2019-10-14
影响因子：
3.1
通讯作者：
Fu, Qiang
中科院分区：
文献类型：
--
作者：
Luo, Zhimin;Xiao, Aiping;Fu, Qiang
黄酮类化合物是中草药中一类重要的生物活性化学成分，从中草药样品中简单、快速、选择性地提取黄酮类化合物具有重要意义。本研究采用分子印迹聚合物与固相萃取（MIPs-SPE）联用技术，建立了一种简单、选择性强的样品前处理方法，用于中草药样品中黄酮类化合物的提取和纯化。以4-乙烯基吡啶为功能单体，乙二醇二甲基丙烯酸酯为交联剂，采用多步溶胀聚合法合成了黄酮类分子印迹聚合物。采用扫描电镜、傅里叶变换红外光谱和元素分析等手段对所得分子印迹聚合物的结构进行了表征。考察了聚合物的识别性能，考察了移动的相的pH值、乙腈的比例、柱温、进样量和移动的流速等因素对识别性能的影响。结果表明，所得分子印迹聚合物具有较好的单分散性，平均粒径约为3-5 μ m。在最佳识别条件下，将所得分子印迹聚合物作为固相萃取的选择性吸附剂，成功地对中草药样品中的黄酮类化合物进行了特异性识别和纯化。
Flavonoids are a class of important bioactive chemical constituents in herbal medicines and the simple, fast and selective extraction of flavonoids from herbal samples is quite meaningful. In this study, a simple and selective sample pretreatment procedure coupling molecularly imprinted polymers and solid-phase extraction (MIPs-SPE) was developed for the extraction and purification of flavonoids from herbal medicine samples. MIPs for flavonoids were synthesized through a multi-step swelling and polymerization method, using 4-vinylpyridine as the functional monomer and ethylene glycol dimethacrylate as the cross-linker. Scanning electron microscopy, Fourier transform infrared spectroscopy and elemental analysis were used to characterize the structural features of the obtained MIPs. The recognition performance of the polymers was investigated and several parameters affecting the recognition performance were also studied including the pH value of the mobile phase, the ratio of acetonitrile, the column temperature, the injection volume and the flow-rate of the mobile phase. The results revealed that a relatively good mono-dispersity of MIPs was obtained, and the average size of the obtained polymers was about 3-5 mu m. Under the optimum recognition conditions, the obtained MIPs were successfully employed as selective adsorbents for SPE for the specific recognition and purification of flavonoids from herbal medicine samples.