Expression of synaptic cell adhesion molecule 1 (SynCAM 1) in different brain regions in a rat subarachnoid hemorrhage model
Expression of synaptic cell adhesion molecule 1 (SynCAM 1) in different brain regions in a rat subarachnoid hemorrhage model
复制标题
大鼠蛛网膜下腔出血模型中不同脑区突触细胞粘附分子1（SynCAM 1）的表达
DOI：
10.1007/s10072-012-1240-5
复制
发表时间：
2013-08
期刊：
影响因子：
--
通讯作者：
G.
中科院分区：
文献类型：
--
作者：
Wang;Z.;Hu;T.;Feng;D.;Chen;G.
突触是神经细胞之间进行交流的连接点，是由突触前和突触后特化的协调组装和紧密附着形成的。突触细胞粘附分子1（SynCAM 1）已被证明是突触功能和行为认知的重要因素。目前的研究旨在调查大鼠实验性蛛网膜下腔出血（SAH）后大脑中 SynCAM 1 的表达。将42只大鼠随机分为7组：对照组、假手术组、第1天组、第3天组、第5天组、第7天组和第14天组。第1天、第3天、第5天、第7天、第14天组均为SAH组，分别于第1、3、5、7、14天处死大鼠。通过将0.3ml新鲜动脉非肝素化血液在20秒内注射到交叉前池中来诱导大鼠SAH模型。进行免疫染色和免疫印迹实验检测SynCAM 1蛋白的表达。 SAH 后第 14 天测量临床行为量表。 SAH组SynCAM 1蛋白表达较假手术组明显降低。 SAH后检测到SynCAM 1表达下调，低潮出现在第1-3天。免疫组织化学染色表明，SynCAM 1 的表达主要存在于所有三个不同区域（例如皮质、海马和小脑）的神经元中。与假手术组大鼠相比，临床行为量表显着下降。我们的结果表明，实验性 SAH 后大脑中 SynCAM 1 的表达下调。这些发现表明 SynCAM 1 表达减少可能会促进 SAH 后认知功能障碍的发生。
Synapses, the junctions between nerve cells through which they communicate, are formed by the coordinated assembly and tight attachment of pre- and postsynaptic specializations. Synaptic cell adhesion molecule 1 (SynCAM 1) has been proved to be an important factor for synapse function and behavior cognition. The current research aimed to investigate the expression of the SynCAM 1 in the brain after experimental subarachnoid hemorrhage (SAH) in rats. A total of 42 rats were randomly divided into seven groups: control group, sham group, day 1, day 3, day 5, day 7, and day 14 groups. Day 1, day 3, day 5, day 7, and day 14 groups were all SAH groups in which the rats were killed on days 1, 3, 5, 7, and 14, respectively. The rat SAH model was induced by injection of 0.3 ml fresh arterial, non-heparinized blood into the prechiasmatic cistern in 20 s. Immunostaining and immunoblotting experiments were performed to detect the expression of SynCAM 1 protein. The clinical behavior scale was measured on day 14 after SAH. The expression of SynCAM 1 protein was decreased remarkably in SAH groups compared with the sham group. The down-regulated expression of SynCAM 1 was detected after SAH and the low ebb was on days 1–3. The immunohistochemical staining demonstrated expression of SynCAM 1 was present mainly in the neurons in all of the three different regions such as cortex, hippocampus, and cerebellum. The clinical behavior scale was significantly decreased compared with sham rats. Our results indicate that SynCAM 1 expression is down-regulated in the brain after experimental SAH. These finding suggests that decreased SynCAM 1 expression may facilitate the development of cognitive dysfunction after SAH.
登录
查看更多内容
影响因子：
2.7
作者：
Silasi, Gergely;Colbourne, Frederick
通讯作者：
Colbourne, Frederick
影响因子：
3.3
作者：
Jeon, H.;Ai, J.;Macdonald, R. L.
通讯作者：
Macdonald, R. L.
影响因子：
4.1
作者：
Mavaddat, N;Sahakian, BJ;Kirkpatrick, PJ
通讯作者：
Kirkpatrick, PJ
影响因子：
4.8
作者：
P. McKenna;J. Willison;B. Phil;Derek Lowe;Glenn Neil-Dwyer
通讯作者：
P. McKenna;J. Willison;B. Phil;Derek Lowe;Glenn Neil-Dwyer
影响因子：
5.3
作者：
Fogel, Adam I.;Akins, Michael R.;Biederer, Thomas
通讯作者：
Biederer, Thomas