Golgicide A reveals essential roles for GBF1 in Golgi assembly and function.
Golgicide A reveals essential roles for GBF1 in Golgi assembly and function.
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DOI：
10.1038/nchembio.144
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发表时间：
2009-03
影响因子：
14.8
通讯作者：
Haslam, David B.
中科院分区：
文献类型：
--
作者：
Saenz, Jose B.;Sun, William J.;Chang, Jae Won;Li, Jinmei;Bursulaya, Badry;Gray, Nathanael S.;Haslam, David B.
腺苷二磷酸核糖基化因子1（Arf 1）在调节真核细胞高尔基体内的分泌运输和膜转运中起着关键作用。Arf 1被鸟嘌呤核苷酸交换因子（ArfGEFs）激活，其赋予囊泡转运的空间和时间特异性。我们在这里描述的发现和特性的杀高尔基体A（GCA），一个有效的，高度特异性的，可逆的抑制剂的顺式高尔基体ArfGEF，GBF 1。GBF 1功能的抑制导致COPI囊泡外壳从高尔基体膜上快速解离，随后高尔基体和反式高尔基体网络（TGN）解体。可溶性和膜相关蛋白的分泌被逮捕在ER-高尔基体中间隔室，而内吞和回收转铁蛋白不受GBF 1抑制。内化的滋贺毒素被逮捕内吞隔室，无法达到分散的TGN。总的来说，这些结果突出了GBF 1在协调双向运输和维持高尔基体结构完整性方面的核心作用。
ADP-ribosylation factor 1 (Arf1) plays a critical role in regulating secretory traffic and membrane transport within the Golgi of eukaryotic cells. Arf1 is activated by guanine nucleotide exchange factors (ArfGEFs) which confer spatial and temporal specificity to vesicular transport. We describe here the discovery and characterization of Golgicide A (GCA), a potent, highly specific, and reversible inhibitor of the cis-Golgi ArfGEF, GBF1. Inhibition of GBF1 function resulted in rapid dissociation of COPI vesicle coat from Golgi membranes and subsequent disassembly of the Golgi and trans-Golgi network (TGN). Secretion of soluble and membrane-associated proteins was arrested at the ER-Golgi intermediate compartment, whereas endocytosis and recycling of transferrin was unaffected by GBF1 inhibition. Internalized shiga toxin was arrested within the endocytic compartment and was unable to reach the dispersed TGN. Collectively, these results highlight the central role for GBF1 in coordinating bidirectional transport and maintaining structural integrity of the Golgi.
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影响因子：
3.3
作者：
Cohen, Lee Ann;Honda, Akira;Donaldson, Julie G.
通讯作者：
Donaldson, Julie G.
影响因子：
11.8
作者：
Macia, Eric;Ehrlich, Marcelo;Kirchhausen, Tomas
通讯作者：
Kirchhausen, Tomas
影响因子：
3.3
作者：
García-Mata, R;Szul, T;Elizabeth, S
通讯作者：
Elizabeth, S
DOI：
10.1083/jcb.109.1.61
发表时间：
1989-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Doms RW;Russ G;Yewdell JW
通讯作者：
Yewdell JW
影响因子：
7.8
作者：
Liu, Wei;Duden, Rainer;Phair, Robert D;Lippincott-Schwartz, Jennifer
通讯作者：
Lippincott-Schwartz, Jennifer