Correct identification of genes from serial analysis of gene expression tag sequences
Correct identification of genes from serial analysis of gene expression tag sequences
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DOI：
10.1006/geno.2002.6730
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发表时间：
2002-04-01
期刊：
影响因子：
4.4
通讯作者：
Wang, SM
中科院分区：
文献类型：
--
作者：
Lee, S;Clark, T;Wang, SM
SAGE（serial analysis of gene expression）是一种全基因组基因表达分析技术。了解SAGE能够在多大程度上准确地指示具有单个标签的基因或表达序列标签（EST）是至关重要的。分析了SAGE标签大小对基因识别的影响。我们的观察表明，SAGE标签一般不足以达到最初设想的识别的唯一性程度。我们的观察还表明，SAGE标签识别基因的局限性可以通过将SAGE标签转化为更长的3 'EST序列，并从SAGE标签中产生更长的cDNA片段用于基因识别（GLGI）方法来克服。
SAGE (serial analysis of gene expression) is a remarkable technique for genome-wide analysis of gene expression. It is crucial to understand the extent to which SAGE can accurately indicate a gene or expressed sequence tag (EST) with a single tag. We analyzed the effect of the size of SAGE tag on gene identification. Our observation indicates that SAGE tags are in general not long enough to achieve the degree of uniqueness of identification originally envisaged. Our observations also indicate that the limitation of using SAGE tag to identify a gene can be overcome by converting SAGE tags into longer 3'EST sequences with the generation of longer cDNA fragments from SAGE tages for gene identification (GLGI) method.